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从长角甲虫幼虫肠道和粪便中分离出的放线菌菌株的特性分析

Characterization of Actinomycetes Strains Isolated from the Intestinal Tract and Feces of the Larvae of the Longhorn Beetle .

作者信息

Santamaría Ramón I, Martínez-Carrasco Ana, Sánchez de la Nieta Ricardo, Torres-Vila Luis M, Bonal Raúl, Martín Jesús, Tormo Rubén, Reyes Fernando, Genilloud Olga, Díaz Margarita

机构信息

Departamento de Microbiología y Genética, Instituto de Biología Funcional y Genómica (IBFG), Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca, C/Zacarías González 2, 37007 Salamanca, Spain.

Servicio de Sanidad Vegetal, Consejería de Agricultura DRPyT, Junta de Extremadura, Avda. Luis Ramallo s/n, 06800 Badajoz, Spain.

出版信息

Microorganisms. 2020 Dec 16;8(12):2013. doi: 10.3390/microorganisms8122013.

Abstract

Actinomycetes constitute a large group of Gram-positive bacteria present in different habitats. One of these habitats involves the association of these bacteria with insects. In this work, we have studied twenty-four actinomycetes strains isolated from the intestinal tract and feces from larvae of the xylophagous coleopteran and have shown that seventeen strains present hydrolytic activity of some of the following substrates: cellulose, hemicellulose, starch and proteins. Fourteen of the isolates produce antimicrobial molecules against the Gram-positive bacteria . Analysis of seven strains led us to identify the production of a wide number of compounds including streptanoate, alpiniamide A, alteramides A and B, coproporphyrin III, deferoxamine, demethylenenocardamine, dihydropicromycin, nocardamine, picromycin, surugamides A, B, C, D and E, tirandamycins A and B, and valinomycin. A significant number of other compounds, whose molecular formulae are not included in the Dictionary of Natural Products (DNP), were also present in the extracts analyzed, which opens up the possibility of identifying new active antibiotics. Molecular identification of ten of the isolated bacteria determined that six of them belong to the genus , two of them are included in the genus and two in the genus

摘要

放线菌是存在于不同生境中的一大类革兰氏阳性细菌。这些生境之一涉及这些细菌与昆虫的关联。在这项工作中,我们研究了从食木鞘翅目幼虫的肠道和粪便中分离出的24株放线菌菌株,并表明17株菌株对以下一些底物具有水解活性:纤维素、半纤维素、淀粉和蛋白质。14株分离物产生针对革兰氏阳性细菌的抗菌分子。对7株菌株的分析使我们鉴定出产生了大量化合物,包括链烷酸、高山酰胺A、互隔交链孢酰胺A和B、粪卟啉III、去铁胺、去甲基诺卡胺、二氢苦霉素、诺卡胺、苦霉素、紫贻贝酰胺A、B、C、D和E、替拉达霉素A和B以及缬氨霉素。在所分析的提取物中还存在大量其他化合物,其分子式未包含在《天然产物词典》(DNP)中,这为鉴定新的活性抗生素提供了可能性。对10株分离细菌的分子鉴定确定其中6株属于 属,2株属于 属,2株属于 属 。 (注:原文中部分属名未给出具体内容)

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d75/7766275/c83114eb9b9a/microorganisms-08-02013-g001.jpg

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