Benhadj Mabrouka, Menasria Taha, Zaatout Nawel, Ranque Stéphane
Biomolecules and Application Laboratory, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University, 12002 Tebessa, Algeria.
Department of Applied Biology, Faculty of Exact Sciences and Natural and Life Sciences, Echahid Cheikh Larbi Tebessi University, 12002 Tebessa, Algeria.
Microorganisms. 2025 Mar 3;13(3):576. doi: 10.3390/microorganisms13030576.
The growing threat of antimicrobial resistance underscores the urgent need to identify new bioactive compounds. In this study, a strain, ACT158, was isolated from a Ramsar wetland ecosystem and found to exhibit broad-spectrum effects against Gram-positive and Gram-negative bacteria, as well as fungal pathogens. The active strain was characterized as according to its morphology, phylogenetic analysis, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH). Whole-genome sequencing (WGS) and annotation revealed a genome size of 6.86 Mb with 5122 coding sequences linked to carbohydrate metabolism, secondary metabolite biosynthesis, and stress responses. Genome mining through antiSMASH revealed 32 biosynthetic gene clusters (BGCs), including those encoding polyketides, nonribosomal peptides, and terpenes, many of which showed low similarity to known clusters. Comparative genomic analysis, showing high genomic synteny with closely related strains. Unique genomic features of ACT158 included additional BGCs and distinct genes associated with biosynthesis pathways and stress adaptation. These findings highlight the strain's potential as a rich source of bioactive compounds and provide insights into its genomic basis for antimicrobial production and its ecological and biotechnological significance.
抗菌药物耐药性的威胁日益增加，凸显了识别新型生物活性化合物的迫切需求。在本研究中，从一处拉姆萨尔湿地生态系统中分离出了一株ACT158菌株，发现其对革兰氏阳性菌、革兰氏阴性菌以及真菌病原体均具有广谱抗菌作用。根据其形态、系统发育分析、平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）对该活性菌株进行了鉴定。全基因组测序（WGS）和注释显示，其基因组大小为6.86 Mb，有5122个编码序列，与碳水化合物代谢、次级代谢产物生物合成及应激反应相关。通过antiSMASH进行基因组挖掘发现了32个生物合成基因簇（BGC），包括那些编码聚酮化合物、非核糖体肽和萜类化合物的基因簇，其中许多与已知基因簇的相似度较低。比较基因组分析表明，ACT158与密切相关菌株具有高度的基因组共线性。ACT158独特的基因组特征包括额外的BGC以及与生物合成途径和应激适应相关的独特基因。这些发现突出了该菌株作为生物活性化合物丰富来源的潜力，并为其抗菌产物的基因组基础及其生态和生物技术意义提供了见解。
