用紫外线技术比较酵母和大肠杆菌中tRNA顺反子的转录连锁关系。
A comparison of transcriptional linkage of tRNA cistrons in yeast and E. coli by the ultraviolet light technique.
作者信息
Feldman H
出版信息
Nucleic Acids Res. 1977 Aug;4(8):2831-41. doi: 10.1093/nar/4.8.2831.
Abstract
The ultraviolet light mapping technique was employed to determine the lengths of tRNA cistrons in yeast. The applicability of the method was first tested in the E. coli system, in which the mapping positions for some tRNA cistrons and the ribosomal 5S RNA genes as well as the existence of multimeric transcription units for tRNAs are known. Rates of the synthesis of the tRNAs and small rRNAs after irradiation with various doses of UV light were determined by pulse labeling and quantitation of the RNA species after twodimensional gel electrophoreses. The small ribosomal RNAs served for internal calibration in the estimtion of the target sizes. Our results suggest that--in contrast to the prokaryotic system--in yeast the majority of the tRNA genes are not linked into transcriptional units.
摘要
采用紫外光定位技术来测定酵母中tRNA顺反子的长度。该方法的适用性首先在大肠杆菌系统中进行了测试，在该系统中，一些tRNA顺反子和核糖体5S RNA基因的定位以及tRNA多聚体转录单位的存在情况是已知的。通过脉冲标记和二维凝胶电泳后对RNA种类进行定量，来测定用不同剂量紫外光照射后tRNA和小rRNA的合成速率。小核糖体RNA用于估计靶标大小的内部校准。我们的结果表明，与原核系统不同，在酵母中，大多数tRNA基因并未连接成转录单位。
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