Departamento de Química, Universidade Federal de Ouro Preto, UFOP, Ouro Preto, 35400-000, Brazil.
Departamento de Química, Universidade Federal de São Carlos, UFSCar, São Carlos, 13565-905, Brazil.
Sci Rep. 2020 Dec 18;10(1):22312. doi: 10.1038/s41598-020-79257-3.
Plants may contain beneficial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass-ionomer-Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass-ionomer cement (GIC) and the composite GIC-BPE were characterized by scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and thermal analysis. The BPE compounds were identified by UPLC-QTOF-MS/MS. The release profile of flavonoids and the mechanical properties of the GIC-BPE composite were assessed. The flavonoids were released through a linear mechanism governing the diffusion for the first 48 h, as evidenced by the M/M relatively to [Formula: see text], at a diffusion coefficient of 1.406 × 10cm s. The ATR-FTIR analysis indicated that a chemical bond between the GIC and BPE components may have occurred, but the compressive strength of GIC-BPE does not differ significantly from that of this glass-ionomer. The GIC-BPE sample revealed an ample bacterial activity at non-cytotoxic concentrations for the human fibroblast MRC-5 cells. These results suggest that the prepared composite may represent an alternative agent for endodontic treatment.
植物可能含有对人类有益或潜在危险的物质。本研究旨在制备和评估一种基于玻璃离子体-巴西胡椒提取物复合材料的新型药物传递系统，以检查其对口腔致病微生物的活性，同时检查其体外生物相容性。用扫描电子显微镜、衰减全反射傅里叶变换红外光谱（ATR-FTIR）和热分析对乙醇巴西胡椒提取物（BPE）、玻璃离子体水泥（GIC）和复合 GIC-BPE 进行了表征。通过 UPLC-QTOF-MS/MS 鉴定了 BPE 化合物。评估了黄酮类化合物的释放曲线和 GIC-BPE 复合材料的机械性能。黄酮类化合物通过扩散控制的线性机制在最初的 48 小时内释放，通过 [Formula: see text] 相对于 M/M 可以证明这一点，扩散系数为 1.406 × 10cm s。ATR-FTIR 分析表明，GIC 和 BPE 成分之间可能发生了化学键合，但 GIC-BPE 的抗压强度与这种玻璃离子体没有显著差异。在对人成纤维细胞 MRC-5 细胞无细胞毒性的浓度下，GIC-BPE 样品显示出对细菌有充分的活性。这些结果表明，所制备的复合材料可能是牙髓治疗的替代剂。
