UHPLC-ESI-QTOF-MS/MS-Based Molecular Networking Guided Isolation and Dereplication of Antibacterial and Antifungal Constituents of .

is an herbal medicine for the treatment of wound infection; therefore this plant may rich in antibacterial agents. UHPLC-ESI-QTOF-MS/MS-Based molecular networking guided isolation and dereplication led to the identification of antibacterial and antifungal agents in . Nine antimicrobial agents in were isolated and characterized; they are divided into four groups including (I) flavonoid glycosides, rhamnazin 3-rhamninoside (), catharticin or rhamnocitrin 3-rhamninoside (), xanthorhamnin B or rhamnetin 3-rhamninoside (), kaempferol 3-rhamninoside () and flavovilloside or quercetin 3-rhamninoside (), (II) benzisochromanquinone, ventilatones B () and A (), (III) a naphthopyrone ventilatone C () and (IV) a triterpene lupeol (). Among the isolated compounds, ventilatone C () was a new compound. Moreover, kaempferol, chrysoeriol, isopimpinellin, rhamnetin, luteolin, emodin, rhamnocitrin, ventilagodenin A, rhamnazin and mukurozidiol, were tentatively identified as antimicrobial compounds in extracts of by a dereplication method. MS fragmentation of rhamnose-containing compounds gave an oxonium ion, CHO at 129, while that of galactose-containing glycosides provided the fragment ion at 163 of CHO. These fragment ions may be used to confirm the presence of rhamnose or galactose in mass spectrometry-based analysis of natural glycosides or oligosaccharide attached to biomolecules, that is, glycoproteins.