基因控制区的研究。VI. 胸腺嘧啶的5-甲基，一种乳糖阻遏物识别位点。
Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site.
作者信息
Goeddel D V, Yansura D G, Caruthers M H
出版信息
Nucleic Acids Res. 1977 Sep;4(9):3039-54. doi: 10.1093/nar/4.9.3039.
Abstract
Three site specific deoxyuridine analogs of lac operator were tested for binding with wild type (SQ) and tight binding (QX86) lac repressors. Insertion of uracil for thymine at site 13 (our nomenclature) significantly reduced the dissociation half-life of QX86 repressor for lac operator DNA (21 vs 1.2 min). Two other sites (6 and 7) are affected to a much lesser extent.
摘要
对乳糖操纵子的三种位点特异性脱氧尿苷类似物与野生型（SQ）和紧密结合型（QX86）乳糖阻遏物的结合情况进行了测试。在13位点（我们的命名法）用尿嘧啶取代胸腺嘧啶显著降低了QX86阻遏物与乳糖操纵子DNA的解离半衰期（21分钟对1.2分钟）。另外两个位点（6和7）受到的影响要小得多。
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