特定序列寡聚脱氧核糖核苷酸的酶促合成。使用含嘧啶类似物的脱氧核糖核苷5'-二磷酸进行多核苷酸磷酸化酶催化的合成。
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed synthesis using pyrimidine analog-containing deoxyribonucleoside 5'-diphosphates.
作者信息
Trip E M, Smith M
出版信息
Nucleic Acids Res. 1978 May;5(5):1539-49. doi: 10.1093/nar/5.5.1539.
Abstract
The E. coli polynucleotide phosphorylase-catalysed reaction of the deoxynucleoside 5'-diphosphates of 5-methyldeoxycytidine, N4-hydroxydeoxycytidine, deoxyuridine and 5-mercurideoxyuridine with the primers d(pT-T-A-G) and d(pT-T-T-T-T-T) have been studied under conditions where the primer is extended, predominantly, by one or two nucleotide residues. In experiments with 5-mercurideoxyuridine 5'-diphosphate, no 5-mercurideoxy-uridine-containing oligonucleotides were produced. The other three nucleotide analogs were found to be good substrates for E. coli PNPase and the conditions established for synthesis with these analogs will allow the construction of a number of biologically useful types of oligodeoxyribonucleotide.
摘要
在引物主要延伸一两个核苷酸残基的条件下，研究了大肠杆菌多核苷酸磷酸化酶催化的5-甲基脱氧胞苷、N4-羟基脱氧胞苷、脱氧尿苷和5-汞脱氧尿苷的脱氧核苷5'-二磷酸与引物d(pT-T-A-G)和d(pT-T-T-T-T-T)的反应。在用5-汞脱氧尿苷5'-二磷酸进行的实验中，未产生含5-汞脱氧尿苷的寡核苷酸。发现其他三种核苷酸类似物是大肠杆菌PNPase的良好底物，用这些类似物建立的合成条件将允许构建多种具有生物学用途的寡脱氧核糖核苷酸类型。
相似文献
1
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed synthesis using pyrimidine analog-containing deoxyribonucleoside 5'-diphosphates.特定序列寡聚脱氧核糖核苷酸的酶促合成。使用含嘧啶类似物的脱氧核糖核苷5'-二磷酸进行多核苷酸磷酸化酶催化的合成。
Nucleic Acids Res. 1978 May;5(5):1539-49. doi: 10.1093/nar/5.5.1539.
2
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.特定序列寡聚脱氧核糖核苷酸的酶促合成。多核苷酸磷酸化酶催化将脱氧核糖核苷酸添加到良好或不良受体的引物上。
Nucleic Acids Res. 1978 May;5(5):1529-38. doi: 10.1093/nar/5.5.1529.
3
Use of E. coli polynucleotide phosphorylase for the synthesis of oligodeoxyribonucleotides of defined sequence.利用大肠杆菌多核苷酸磷酸化酶合成特定序列的寡脱氧核糖核苷酸。
Methods Enzymol. 1980;65(1):687-701. doi: 10.1016/s0076-6879(80)65067-8.
4
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis.特定序列脱氧核糖寡核苷酸的酶促合成。脱氧核糖寡核苷酸合成。
Nucleic Acids Res. 1974 Dec;1(12):1649-64. doi: 10.1093/nar/1.12.1649.
5
Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence.特定序列寡脱氧核糖核苷酸的酶促合成。
J Biol Chem. 1978 Apr 25;253(8):2532-9.
6
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.特定序列脱氧核糖寡核苷酸的酶促合成。该酶的性质。
Nucleic Acids Res. 1974 Dec;1(12):1631-47. doi: 10.1093/nar/1.12.1631.
7
Enzymatic synthesis of oligonucleotides of defined sequence. The "single addition" of 2(3)-O-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase.特定序列寡核苷酸的酶促合成。嗜热多核苷酸磷酸化酶催化2(3)-O-二氢肉桂酰核苷5'-二磷酸向引物寡核苷酸的“单次添加”。
J Biochem. 1976 Jul;80(1):33-7. doi: 10.1093/oxfordjournals.jbchem.a131254.
8
Specific oligoribonucleotide synthesis using primer-independent polynucleotide phosphorylase.使用不依赖引物的多核苷酸磷酸化酶进行特定寡核糖核苷酸的合成。
Prep Biochem. 1975;5(5-6):423-32. doi: 10.1080/00327487508061589.
9
2'-O-(alpha-methoxyethyl)nucleoside 5'-diphosphates as "single-addition" substrates in the synthesis of specific oligoribonucleotides with polynucleotide phosphorylase.2'-O-(α-甲氧基乙基)核苷5'-二磷酸作为“单加成”底物用于用多核苷酸磷酸化酶合成特定的寡核糖核苷酸。
Biochemistry. 1973 Sep 25;12(20):3956-62. doi: 10.1021/bi00744a027.
10
Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.特定序列寡核苷酸的酶促合成：酵母异-1-细胞色素c基因片段的合成
Proc Natl Acad Sci U S A. 1977 Jan;74(1):96-100. doi: 10.1073/pnas.74.1.96.
引用本文的文献
1
T4 RNA ligase catalyzed synthesis of base analogue-containing oligodeoxyribonucleotides and a characterization of their thermal stabilities.T4 RNA连接酶催化含碱基类似物的寡脱氧核糖核苷酸的合成及其热稳定性的表征。
Nucleic Acids Res. 1985 Dec 20;13(24):8665-84. doi: 10.1093/nar/13.24.8665.
本文引用的文献
1
SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE.通过阴离子交换合成核苷酸酐。
Biochim Biophys Acta. 1964 Sep 11;91:1-13. doi: 10.1016/0926-6550(64)90164-1.
2
Rapid ion-exchange chromatographic microanalysis of ultraviolet-absorbing materials and its application to nucleosides.紫外吸收物质的快速离子交换色谱微分析及其在核苷中的应用。
Anal Biochem. 1968 Oct 24;25(1):77-98. doi: 10.1016/0003-2697(68)90083-3.
3
The interaction of actinomycin with DNA: requirement for the 2-amino group of purines.放线菌素与DNA的相互作用：嘌呤2-氨基基团的必要性。
Proc Natl Acad Sci U S A. 1967 Apr;57(4):1036-42. doi: 10.1073/pnas.57.4.1036.
4
Site-directed mutagenesis: generation of an extracistronic mutation in bacteriophage Q beta RNA.定点诱变：噬菌体Qβ RNA中顺反子外突变的产生
J Mol Biol. 1974 Oct 25;89(2):255-72. doi: 10.1016/0022-2836(74)90517-8.
5
Mechanism of the mutagenic action of hydroxylamine. VII. Functional activity and specificity of cytidine triphosphate modified with hydroxylamine and O-methylhydroxylamine.羟胺的诱变作用机制。VII. 经羟胺和O-甲基羟胺修饰的三磷酸胞苷的功能活性及特异性。
Biochim Biophys Acta. 1972 Dec 6;287(2):195-210. doi: 10.1016/0005-2787(72)90370-x.
6
The determination of 2'-O-methylnucleosides in RNA.RNA中2'-O-甲基核苷的测定
Anal Biochem. 1974 Oct;61(2):367-78. doi: 10.1016/0003-2697(74)90404-7.
7
A rapid separation of the four major deoxynucleosides and deoxyinosine by high-pressure liquid cation-exchange chromatography.通过高压液相阳离子交换色谱法快速分离四种主要脱氧核苷和脱氧肌苷。
Anal Biochem. 1973 Aug;54(2):346-52. doi: 10.1016/0003-2697(73)90362-x.
8
Direct covalent mercuration of nucleotides and polynucleotides.核苷酸和多核苷酸的直接共价汞化。
Biochemistry. 1975 Jun 3;14(11):2447-57. doi: 10.1021/bi00682a027.
9
Structural and energetic consequences of noncomplementary base oppositions in nucleic acid helices.核酸螺旋中非互补碱基配对的结构和能量后果。
Prog Nucleic Acid Res Mol Biol. 1975;15(0):185-218. doi: 10.1016/s0079-6603(08)60120-8.
10
A guanosine to adenosine transition in the 3' terminal extracistronic region of bacteriophage Q beta RNA leading to loss of infectivity.噬菌体Qβ RNA 3' 末端顺反子外区域中鸟苷到腺苷的转变导致感染性丧失。
J Mol Biol. 1977 May 15;112(2):235-52. doi: 10.1016/s0022-2836(77)80141-1.
Zotero 插件