高通量生产血小板样颗粒。
High-Throughput Production of Platelet-Like Particles.
机构信息
Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA.
出版信息
Methods Mol Biol. 2021;2258:273-283. doi: 10.1007/978-1-0716-1174-6_18.
Abstract
The in vitro production of platelets could provide a life-saving intervention for patients that would otherwise require donor-derived platelets. Producing large numbers of platelets in vitro from their progenitor cells, megakaryocytes, remains remarkably difficult and inefficient. Here, a human megakaryoblast leukemia cell line (MEG-01) was used to assess the maturation of megakaryocytes and to develop a new methodology for producing high numbers of platelet-like particles from mature MEG-01 cells in vitro.
摘要
在体外生产血小板可以为那些否则需要供体来源的血小板的患者提供救命的干预措施。从其祖细胞巨核细胞体外大量生产血小板仍然非常困难和低效。在这里，使用人巨核母细胞白血病细胞系（MEG-01）来评估巨核细胞的成熟，并开发一种从体外成熟的 MEG-01 细胞中产生大量血小板样颗粒的新方法。
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