基于铽(III)的氮掺杂碳点用于海鲜中汞(II)的比率荧光传感。

Terbium (III)-referenced N-doped carbon dots for ratiometric fluorescent sensing of mercury (II) in seafood.

机构信息

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.

College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling, Shaanxi 712100, PR China; National Engineering Research Center of Agriculture Integration Test (Yangling), Yangling, Shaanxi 712100, PR China.

出版信息

Food Chem. 2020 Aug 1;320:126624. doi: 10.1016/j.foodchem.2020.126624. Epub 2020 Mar 16.

DOI:10.1016/j.foodchem.2020.126624

PMID:32208181

Abstract

A ratiometric fluorescence (FL) sensor was fabricated by coordinating 2, 6-pyridinedicarboxylic acid (DPA) sensitized Tb (Tb-DPA) with NH and COOH on the surface of the N-doped carbon dots (N-CDs) for detecting Hg in seafood. The sensor exhibited two FL emissions at 436 nm (N-CDs) as the response signal and at 543 nm (Tb-DPA) as the reference signal when excited at 290 nm. After adding Hg, the FL emission at 436 nm was significantly quenched and the FL emission at 543 nm was negligibly changed. The electron transfer (ET) between COO of N-CDs and Hg led to the FL quenching of N-CDs. The FL ratio (F/F) exhibited a good linear relationship in the Hg concentrations of 1161.51 μM with a low limit of detection (LOD) of ~37 nM. The sensor presented high selectivity, satisfactory accuracy and precision toward Hg in seafood with recoveries of 86.45-114.47% and RSDs of 0.20-1.92%.

摘要

一个比率荧光（FL）传感器是通过协调 2,6-吡啶二甲酸（DPA）敏化的 Tb（Tb-DPA）与 NH 和 COOH 在 N 掺杂的碳点（N-CDs）的表面来检测海鲜中的 Hg 而制成的。该传感器在 290nm 激发时，在 436nm（N-CDs）处显示两个 FL 发射，作为响应信号，在 543nm（Tb-DPA）处显示作为参考信号。加入 Hg 后，在 436nm 的 FL 发射显著猝灭，而在 543nm 的 FL 发射几乎不变。N-CDs 和 Hg 之间的 COO 的电子转移（ET）导致 N-CDs 的 FL 猝灭。FL 比（F/F）在 Hg 浓度为 1161.51μM 时表现出良好的线性关系，检测限（LOD）约为~37nM。该传感器对海鲜中的 Hg 具有高选择性、令人满意的准确性和精密度，回收率为 86.45-114.47%，RSD 为 0.20-1.92%。