Cipriani Paolo, Palomba Marialetizia, Giulietti Lucilla, Bao Miguel, Mattiucci Simonetta, Levsen Arne
Department of Contaminants and Biohazards, Institute of Marine Research (IMR), Nordnes, Bergen, Norway.
Department of Public Health and Infectious Diseases, Section of Parasitology, Sapienza - University of Rome, Italy.
Int J Food Microbiol. 2021 Feb 2;339:109021. doi: 10.1016/j.ijfoodmicro.2020.109021. Epub 2020 Dec 11.
Few reports exist upon the occurrence and localization of zoonotic anisakid nematodes in T. sagittatus, especially in the mantle of the squid. The occurrence and site of infection of larval anisakids in 98 T. sagittatus caught West off St. Kilda, NE Atlantic Ocean, were investigated. Squids were examined for anisakids using the UV-Press method. In total, 689 nematodes were detected in the viscera and mantle. According to morphology, all the larvae (L) were assigned to genus Anisakis. Diagnostic allozymes and mtDNA cox2 sequence analysis permitted to genetically identify all larvae as Anisakis simplex (s.s.) (N = 100). Overall prevalence (P = 81%) and mean intensity (mI = 8.6) of infection with A. simplex are provided. Most of the larvae present in the mantle cavity were embedded in the stomach wall or attached in the outer layer of the stomach and caecum (49%). Over a third of squids (37%) hosted A. simplex (s.s.) larvae in the mantle. A novel schematized representation of larvae distribution in the mantle is provided, showing where they were mostly located. According to the results obtained, the risk of anisakiasis associated with consumption of raw or undercooked T. sagittatus should be considered.
关于箭乌贼（T. sagittatus）体内人兽共患异尖线虫的发生情况及定位，尤其是在乌贼外套膜中的情况，相关报道较少。本研究调查了在东北大西洋圣基尔达以西捕获的98只箭乌贼幼虫期异尖线虫的感染情况及感染部位。采用紫外线按压法对乌贼进行异尖线虫检查。在内脏和外套膜中共检测到689条线虫。根据形态学特征，所有幼虫均归为异尖线虫属（Anisakis）。通过诊断性等位酶和线粒体DNA细胞色素氧化酶亚基2（mtDNA cox2）序列分析，从基因上鉴定所有幼虫均为简单异尖线虫（Anisakis simplex）（s.s.）（N = 100）。给出了简单异尖线虫的总体感染率（P = 81%）和平均感染强度（mI = 8.6）。外套膜腔中的大多数幼虫嵌入胃壁或附着在胃和盲肠的外层（49%）。超过三分之一的乌贼（37%）在外套膜中寄生有简单异尖线虫（s.s.）幼虫。提供了一个关于幼虫在外套膜中分布的新颖示意图，展示了它们最主要的分布位置。根据所得结果，应考虑食用生的或未煮熟的箭乌贼所带来的异尖线虫病风险。
