在B - 30位特异性标记有氚的胰岛素的酶促半合成。
Enzymatic semisynthesis of insulin specifically labelled with tritium at position B-30.
作者信息
Davies J G, Rose K, Bradshaw C G, Offord R E
机构信息
Departement de Biochimie Médicale, Centre Médical-Universitaire, Genève, Switzerland.
出版信息
Protein Eng. 1987 Oct-Nov;1(5):407-11. doi: 10.1093/protein/1.5.407.
PMID:3334095
Abstract
We have synthesized porcine insulin labelled with tritium at position B-30 using enzyme-catalysed formation of a peptide bond. The resulting insulin derivative has the label in the expected position and is biologically active. We have tested our procedure to prepare batches up to 50 muCi of tritiated insulin at a specific radioactivity of up to 1.14 Ci/mmol.
摘要
我们利用酶催化肽键形成反应合成了在B - 30位用氚标记的猪胰岛素。所得胰岛素衍生物的标记位置符合预期且具有生物活性。我们已经测试了我们的方法，能够制备高达50微居里的氚标记胰岛素批次，其比活度高达1.14居里/毫摩尔。
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