大鼠循环中源自[[3H]苯丙氨酸B1]胰岛素的放射性产物的化学特征分析。
The chemical characterization of the radioactive products derived from [[3H]PheB1]insulin in the circulation of the rat.
作者信息
Davies J G, Offord R E
机构信息
Département de Biochimie Médicale, Centre Médical Universitaire, Genève, Switzerland.
出版信息
Biochem J. 1988 Mar 1;250(2):389-93. doi: 10.1042/bj2500389.
Abstract
- Gel filtration of rat plasma taken 1 h after subcutaneous injection of [[3H]PheB1]insulin gives three peaks of radioactivity. 2. The material in these peaks was characterized by electrophoresis and chromatography. 3. We conclude that [[3H]PheB1]insulin is rapidly degraded to free tritiated phenylalanine. The phenylalanine is subsequently used for synthesis of plasma proteins de novo.
摘要
- 皮下注射[[3H]苯丙氨酸B1]胰岛素1小时后采集的大鼠血浆经凝胶过滤产生三个放射性峰。2. 通过电泳和色谱法对这些峰中的物质进行了表征。3. 我们得出结论，[[3H]苯丙氨酸B1]胰岛素迅速降解为游离的氚化苯丙氨酸。随后苯丙氨酸被用于从头合成血浆蛋白。
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