制备比放射性高达每毫摩尔20居里的半合成氚化胰岛素。
The preparation of a semisynthetic tritiated insulin with a specific radioactivity of up to 20 Curies per millimole.
作者信息
Halban P A, Offord R E
出版信息
Biochem J. 1975 Nov;151(2):219-25. doi: 10.1042/bj1510219.
Abstract
- This paper describes the semisynthesis of [3H]insulin of high specific radioactivity which is indistinguishable from native insulin in its hypoglycaemic activity towards mice. 2. The maximum specific radioactivity so far obtained is 20 Ci/mmol. At this value the recommended scale of preparation is between 1 mCi (30 mug of product) and 10mCi (300 mug of product). Reaction volumes are then of the order of 10 mul. 3. Special problems arise when the techniques of peptide synthesis are applied to the production of such small quantities of material. We describe these problems and discuss the means of overcoming them.
摘要
- 本文描述了高比放射性[3H]胰岛素的半合成，其对小鼠的降血糖活性与天然胰岛素无差异。2. 目前获得的最大比放射性为20居里/毫摩尔。在此值下，推荐的制备规模为1毫居里（30微克产物）至10毫居里（300微克产物）。反应体积约为10微升。3. 将肽合成技术应用于如此少量物质的生产时会出现特殊问题。我们描述了这些问题并讨论了克服它们的方法。
相似文献
1
The preparation of a semisynthetic tritiated insulin with a specific radioactivity of up to 20 Curies per millimole.制备比放射性高达每毫摩尔20居里的半合成氚化胰岛素。
Biochem J. 1975 Nov;151(2):219-25. doi: 10.1042/bj1510219.
2
The preparation of tritiated insulin specifically labelled by semisynthesis at glycine-A1.在甘氨酸 -A1 处通过半合成特异性标记的氚化胰岛素的制备。
Biochem J. 1985 Oct 15;231(2):389-92. doi: 10.1042/bj2310389.
3
Improved preparation of semisynthetic PheB1-tritiated insulin.半合成苯丙氨酸B1-氚标记胰岛素的改进制备方法。
Biol Chem Hoppe Seyler. 1987 Mar;368(3):239-48. doi: 10.1515/bchm3.1987.368.1.239.
4
Preparation of tritiated alpha-melanotropin with high specific radioactivity.
Int J Pept Protein Res. 1981 Apr;17(4):514-8. doi: 10.1111/j.1399-3011.1981.tb02022.x.
5
Binding and degradation of semisynthetic tritiated insulin by IM-9 cultured human lymphocytes.
Diabetes. 1980 Sep;29(9):730-5. doi: 10.2337/diab.29.9.730.
6
Synthesis of tritiated rat insulin with high specific activity: a method for radiolabeling the active site of insulin.高比活度氚标记大鼠胰岛素的合成：一种对胰岛素活性位点进行放射性标记的方法。
Biochem Biophys Res Commun. 1981 Apr 30;99(4):1058-64. doi: 10.1016/0006-291x(81)90726-9.
7
Studies of the biological and immunological properties of parathyroid hormone, labeled selectively on the methionine residues by [3H]methyl exchange to high specific activity.
Endocrinology. 1979 Oct;105(4):1020-30. doi: 10.1210/endo-105-4-1020.
8
The degradation of semisynthetic tritiated insulin by perfused mouse livers.灌注小鼠肝脏对半合成氚标记胰岛素的降解作用。
Biochem J. 1976 Nov 15;160(2):409-12. doi: 10.1042/bj1600409.
9
Enzymatic semisynthesis of insulin specifically labelled with tritium at position B-30.在B - 30位特异性标记有氚的胰岛素的酶促半合成。
Protein Eng. 1987 Oct-Nov;1(5):407-11. doi: 10.1093/protein/1.5.407.
10
Monomeric B27 Lys destripeptide insulin: semisynthesis, characterization and biological activity.单体B27赖氨酸去条纹肽胰岛素：半合成、表征及生物活性
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Mar;35(3):215-8.
引用本文的文献
1
Kidney function, β-cell function and glucose tolerance in older men.老年男性的肾功能、β细胞功能与葡萄糖耐量
J Clin Endocrinol Metab. 2015 Feb;100(2):587-93. doi: 10.1210/jc.2014-3313. Epub 2014 Nov 27.
2
Demonstration of a relatively hepatoselective effect of covalent insulin dimers on glucose metabolism in dogs.共价胰岛素二聚体对犬葡萄糖代谢的相对肝选择性作用的证明。
Diabetologia. 1995 Sep;38(9):1007-13. doi: 10.1007/BF00402169.
3
Preparation and characterization of novel substrates of insulin proteinase (EC 3.4.99.45).胰岛素蛋白酶（EC 3.4.99.45）新型底物的制备与表征
Biochem J. 1994 Sep 15;302 ( Pt 3)(Pt 3):907-11. doi: 10.1042/bj3020907.
4
Increased clearance and degradation of [3H]insulin in streptozotocin diabetic rats.链脲佐菌素诱导的糖尿病大鼠中[3H]胰岛素清除率及降解率的增加
J Clin Invest. 1981 Mar;67(3):673-80. doi: 10.1172/JCI110082.
5
The preparation of tritiated insulin specifically labelled by semisynthesis at glycine-A1.在甘氨酸 -A1 处通过半合成特异性标记的氚化胰岛素的制备。
Biochem J. 1985 Oct 15;231(2):389-92. doi: 10.1042/bj2310389.
6
Identification of some cleavage sites of insulin by insulin proteinase.胰岛素蛋白酶对胰岛素某些裂解位点的鉴定。
Biochem J. 1986 Dec 1;240(2):609-12. doi: 10.1042/bj2400609.
7
The identification of a major product of the degradation of insulin by 'insulin proteinase' (EC 3.4.22.11).通过“胰岛素蛋白酶”（EC 3.4.22.11）鉴定胰岛素降解的主要产物。
Biochem J. 1986 Aug 1;237(3):631-7. doi: 10.1042/bj2370631.
8
The chemical characterization of the radioactive products derived from [[3H]PheB1]insulin in the circulation of the rat.大鼠循环中源自[[3H]苯丙氨酸B1]胰岛素的放射性产物的化学特征分析。
Biochem J. 1988 Mar 1;250(2):389-93. doi: 10.1042/bj2500389.
9
Identification of radioactive insulin fragments liberated by insulin proteinase during the degradation of semisynthetic [3H]GlyA1]insulin and [3H]PheB1]insulin.在半合成的[3H]甘氨酸A1胰岛素和[3H]苯丙氨酸B1胰岛素降解过程中，鉴定胰岛素蛋白酶释放的放射性胰岛素片段。
Biochem J. 1988 Jan 1;249(1):209-14. doi: 10.1042/bj2490209.
10
The degradation of semisynthetic tritiated insulin by perfused mouse livers.灌注小鼠肝脏对半合成氚标记胰岛素的降解作用。
Biochem J. 1976 Nov 15;160(2):409-12. doi: 10.1042/bj1600409.
本文引用的文献
1
[Improved synthesis of etert-butyloxycarbonyl-amino acids through pH stable reactions].[通过pH稳定反应改进叔丁氧羰基氨基酸的合成]
Justus Liebigs Ann Chem. 1967;702:188-96. doi: 10.1002/jlac.19677020123.
2
Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes.单碘胰岛素：其生物活性及与脂肪细胞和肝细胞膜结合的证明
Biochem Biophys Res Commun. 1971 Apr 16;43(2):400-8. doi: 10.1016/0006-291x(71)90767-4.
3
Protected intermediate for the preparation of semisynthetic insulins.用于制备半合成胰岛素的保护中间体。
Nature. 1970 Aug 15;227(5259):716-8. doi: 10.1038/227716a0.
4
[Bis (t-butyloxycarbonyl) insulin].[双（叔丁氧羰基）胰岛素]
Hoppe Seylers Z Physiol Chem. 1971 Nov;352(11):1487-90. doi: 10.1515/bchm2.1971.352.2.1487.
5
The preparation and evaluation of tritiated polyalanyl insulin derivatives.氚标记聚丙氨酰胰岛素衍生物的制备与评价
Endocrinology. 1972 Jan;90(1):112-22. doi: 10.1210/endo-90-1-112.
6
7
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
8
The use of semisynthetically-introduced 13 C probes for nuclear magnetic resonance studies on insulin.用于胰岛素核磁共振研究的半合成引入的¹³C探针的应用。
FEBS Lett. 1972 Oct 1;26(1):286-8. doi: 10.1016/0014-5793(72)80594-5.
9
Membrane receptors.膜受体
Annu Rev Biochem. 1974;43(0):169-214. doi: 10.1146/annurev.bi.43.070174.001125.
10
Insulin receptors.胰岛素受体
Annu Rev Med. 1973;24:233-40. doi: 10.1146/annurev.me.24.020173.001313.
Zotero 插件