制备比放射性高达每毫摩尔20居里的半合成氚化胰岛素。
The preparation of a semisynthetic tritiated insulin with a specific radioactivity of up to 20 Curies per millimole.
作者信息
Halban P A, Offord R E
出版信息
Biochem J. 1975 Nov;151(2):219-25. doi: 10.1042/bj1510219.
Abstract
- This paper describes the semisynthesis of [3H]insulin of high specific radioactivity which is indistinguishable from native insulin in its hypoglycaemic activity towards mice. 2. The maximum specific radioactivity so far obtained is 20 Ci/mmol. At this value the recommended scale of preparation is between 1 mCi (30 mug of product) and 10mCi (300 mug of product). Reaction volumes are then of the order of 10 mul. 3. Special problems arise when the techniques of peptide synthesis are applied to the production of such small quantities of material. We describe these problems and discuss the means of overcoming them.
摘要
- 本文描述了高比放射性[3H]胰岛素的半合成，其对小鼠的降血糖活性与天然胰岛素无差异。2. 目前获得的最大比放射性为20居里/毫摩尔。在此值下，推荐的制备规模为1毫居里（30微克产物）至10毫居里（300微克产物）。反应体积约为10微升。3. 将肽合成技术应用于如此少量物质的生产时会出现特殊问题。我们描述了这些问题并讨论了克服它们的方法。
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本文引用的文献
1
[Improved synthesis of etert-butyloxycarbonyl-amino acids through pH stable reactions].[通过pH稳定反应改进叔丁氧羰基氨基酸的合成]
Justus Liebigs Ann Chem. 1967;702:188-96. doi: 10.1002/jlac.19677020123.
2
Monoiodoinsulin: demonstration of its biological activity and binding to fat cells and liver membranes.单碘胰岛素：其生物活性及与脂肪细胞和肝细胞膜结合的证明
Biochem Biophys Res Commun. 1971 Apr 16;43(2):400-8. doi: 10.1016/0006-291x(71)90767-4.
3
Protected intermediate for the preparation of semisynthetic insulins.用于制备半合成胰岛素的保护中间体。
Nature. 1970 Aug 15;227(5259):716-8. doi: 10.1038/227716a0.
4
[Bis (t-butyloxycarbonyl) insulin].[双（叔丁氧羰基）胰岛素]
Hoppe Seylers Z Physiol Chem. 1971 Nov;352(11):1487-90. doi: 10.1515/bchm2.1971.352.2.1487.
5
The preparation and evaluation of tritiated polyalanyl insulin derivatives.氚标记聚丙氨酰胰岛素衍生物的制备与评价
Endocrinology. 1972 Jan;90(1):112-22. doi: 10.1210/endo-90-1-112.
6
7
The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.通过与含¹²⁵I的酰化剂结合将蛋白质标记至高比放射性。
Biochem J. 1973 Jul;133(3):529-39. doi: 10.1042/bj1330529.
8
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FEBS Lett. 1972 Oct 1;26(1):286-8. doi: 10.1016/0014-5793(72)80594-5.
9
Membrane receptors.膜受体
Annu Rev Biochem. 1974;43(0):169-214. doi: 10.1146/annurev.bi.43.070174.001125.
10
Insulin receptors.
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