Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica (IUNAN), Universidad de Córdoba, Campus de Rabanales, Edificio Anexo "Marie Curie", Córdoba E-14071, España.
Departamento de Química Analítica, Instituto Universitario de Investigación en Química Fina y Nanoquímica (IUNAN), Universidad de Córdoba, Campus de Rabanales, Edificio Anexo "Marie Curie", Córdoba E-14071, España.
J Chromatogr A. 2021 Jan 11;1636:461798. doi: 10.1016/j.chroma.2020.461798. Epub 2020 Dec 10.
Liposomes, mainly formed by phospholipids and cholesterol that entrapped different compounds, were separated and characterized using asymmetric flow field-flow fractionation (AF4) coupled with a multi-angle light scattering detector (MALS). AF4 allows the separation of liposomes according to their hydrodynamic size, and the particle size can be estimated directly by their elution time. Besides, different synthesized liposome suspensions of liposomes with different species encapsulated in different places in liposomes were prepared with analytical purposes to be studied. These liposomes were: empty liposomes (e-Ls), magnetoliposomes (MLs) with FeO@AuNPs-C12SH inside the lipid bilayer, and long-wavelength fluorophores encapsulated into the aqueous cavity of liposomes (Ls-LWF). The optimization process of the variables that affect the fractionation has been established. The separation effectiveness has been compared with the results achieved with a photon-correlation spectroscopy analyzer based on dynamic light scattering (DLS) and transmission electron microscopy (TEM), used in self-assembly structures characterization. In all cases, three different classes of liposomes have been obtained; two are commonly appaired in all studied samples, while only a third class is characteristic for each of the liposomes. This mean that the proposed methodology could be used for identifying liposomes according to the encapsulated material.
脂质体主要由磷脂和胆固醇组成，可包封不同的化合物，使用不对称流场流分离（AF4）与多角度光散射检测器（MALS）进行分离和表征。AF4 根据其流体动力学大小分离脂质体，并且可以通过它们的洗脱时间直接估计粒径。此外，为了进行研究，还制备了具有不同物种包封在脂质体不同位置的分析目的的不同合成脂质体悬浮液。这些脂质体是：空脂质体（e-Ls）、脂质双层内包裹 FeO@AuNPs-C12SH 的磁脂质体（MLs）以及长波长荧光团包封在脂质体水腔中的脂质体（Ls-LWF）。已经建立了影响分馏的变量的优化过程。已经将分离效率与基于动态光散射（DLS）和透射电子显微镜（TEM）的光子相关光谱分析仪的结果进行了比较，用于自组装结构的表征。在所有情况下，都获得了三种不同类型的脂质体；两种在所有研究样品中都常见，而只有第三种脂质体是每种脂质体的特征。这意味着所提出的方法可用于根据包封材料识别脂质体。
