El-Sharkawy Yasser H, Elbasuney Sherif, Radwan Sara M, Askar Mostafa A, El-Sayyad Gharieb S
Head of Biomedical Engineering Department, Military Technical College Cairo Egypt.
Head of Nanotechnology Research Center, Military Technical College Cairo Egypt
RSC Adv. 2021 Oct 11;11(53):33319-33325. doi: 10.1039/d1ra05599b. eCollection 2021 Oct 8.
Different cancers are caused by accumulation of numerous genetic and epigenetic changes. Recently, nonlinear polarization has been considered as a marvelous tool for several medical applications. The capability of nonlinear polarization, to monitor any changes in RNA's spectral signature due to breast cancer (BC) was evaluated. Blood samples, from healthy controls and BC patients, were collected for whole blood preparation for genomic total RNA purification. Total RNA samples were stimulated with a light-emitting diode (LED) source of 565 nm; the resonance frequency of investigated RNA samples was captured and processed hyperspectral imaging. Resonance frequency signatures were processed using fast Fourier transform in an attempt to differentiate between RNA (control) and RNA (BC) frequency response. RNA (BC) demonstrated a characteristic signal at 0.02 GHz, as well as a phase shift at 0.031, and 0.070 GHZ from RNA (control). These features could offer early BC diagnosis. This is the first time to describe an optical methodology based on nonlinear polarization as a facile principle to distinguish and identify RNA alterations in BC by their characteristic fingerprint spectral signature.
不同的癌症是由众多基因和表观遗传变化的积累所引起的。最近，非线性极化已被视为一种用于多种医学应用的奇妙工具。评估了非线性极化监测由于乳腺癌（BC）导致的RNA光谱特征变化的能力。收集来自健康对照者和BC患者的血液样本，用于制备全血以纯化基因组总RNA。用565nm的发光二极管（LED）光源刺激总RNA样本；捕获并处理所研究RNA样本的共振频率进行高光谱成像。使用快速傅里叶变换处理共振频率特征，试图区分RNA（对照）和RNA（BC）的频率响应。RNA（BC）在0.02GHz处显示出特征信号，并且相对于RNA（对照）在0.031GHz和0.070GHz处有相移。这些特征可为早期BC诊断提供依据。这是首次描述一种基于非线性极化的光学方法，作为一种简便的原理，通过其特征指纹光谱特征来区分和识别BC中的RNA改变。
