Laboratory of Veterinary Microbiology, Center of Agrarian Sciences, Federal Rural University of the Semi-Arid Region, Mossoró, Rio Grande do Norte, Brazil.
Laboratory of Animal Biotechnology, Center for Biological and Health Sciences, Federal Rural University of Semi-arid Region, UFERSA, Mossoró, RN, Brazil.
J Appl Microbiol. 2021 Aug;131(2):604-614. doi: 10.1111/jam.14977. Epub 2021 Jan 7.
To evaluate the applicability of the Mimosa tenuiflora and Eucalyptus urograndis pyroligneous acids (PAs) as alternative antiseptics in dairy goats.
Cytotoxicity was evaluated in vitro using bacteria, as well as in vivo using goats, and the influence of PAs on the physicochemical parameters of fresh milk were examined. The cytotoxicity of PAs was evaluated in terms of morphology, cell viability and metabolic activity of goat tegumentary cells. The PA of M. tenuiflora had results similar to those of 2% iodine. For the in vitro tests, strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were used with the well technique, demonstrating inhibition halos greater than 9 mm. In the in vivo test, 15 animals were used per phase of the experiment, and the plate counting technique showed that there was antiseptic action of both extracts, with emphasis on the M. tenuiflora PA. Physicochemical analysis of the milk showed that neither PAs interfered with its physical-chemical parameters.
The PA of M. tenuiflora presented potential as an alternative antiseptic in dairy goats.
This study demonstrates the use of PA as an antimicrobial agent in animals.
评估金合欢和桉树焦木醋酸（PA）作为奶山羊替代防腐剂的适用性。
体外使用细菌，体内使用山羊评估 PA 的细胞毒性，并检查 PA 对新鲜牛奶理化参数的影响。通过山羊表皮细胞的形态、细胞活力和代谢活性来评估 PA 的细胞毒性。金合欢 PA 的结果与 2%碘相似。对于体外试验，使用了金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌的菌株，采用孔法技术，显示抑制晕大于 9mm。在体内试验中，每个试验阶段使用 15 只动物，平板计数技术表明两种提取物均具有防腐作用，金合欢 PA 的作用更为明显。牛奶的理化分析表明，PA 均未干扰其理化参数。
金合欢 PA 具有作为奶山羊替代防腐剂的潜力。
本研究证明了 PA 作为动物抗菌剂的用途。
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