da Silva Bismark Alves, Feijó Francisco Marlon Carneiro, Alves Nilza Dutra, Pimenta Alexandre Santos, Benicio Leon Denner Moreira, da Silva Júnior Enilson Claudio, Santos Caio Sérgio, Pereira Alexsandra Fernandes, Moura Yasmin Beatriz França, Gama Gil Sander Prospero, Neto Evilasio de Souza Lima
Laboratory of Veterinary Microbiology, Center of Agrarian Sciences, Federal Rural University of Semi-Arid Region - UFERSA, Mossoró, Brazil.
Laboratory of Veterinary Microbiology, Center of Agrarian Sciences, Federal Rural University of Semi-Arid Region - UFERSA, Mossoró, Brazil.
Vet Microbiol. 2023 Apr;279:109670. doi: 10.1016/j.vetmic.2023.109670. Epub 2023 Jan 27.
Bovine mastitis is one of the most frequent diseases in dairy cattle worldwide. The use of antiseptics in milking, if properly used, can lead to a reduction in potentially pathogenic microorganisms and their transmission between herds. Several medicinal plants have antiseptic potential, including eucalyptus (Eucalyptus spp.). Therefore, the objective of this study was to evaluate the effectiveness of wood vinegar from Eucalyptus urograndis clone GG I144 (EU) as an antiseptic in vitro and in vivo; in addition, to its cytotoxicity and antimicrobial resistance. Fifteen bovines were used, lactating females 3-6 years of age and divided into three groups of five animals each. The wood vinegar was placed in the teats of the animal for 28 days and collections of cellular debris were performed every 7 days. At the Veterinary Microbiology Laboratory (LAMIV) of UFERSA, the samples were processed and serial dilution was performed in Petri plates with plate count agar (PCA) at 37 °C. Cytotoxicity was verified based on morphological alterations and metabolic activity. Morphological changes were not observed in all cells incubated with 1 % pyroligneous extract. The in vitro data demonstrated antimicrobial activity against S. aureus, S. agalactiae, Salmonella, E. coli and P. aeruginosa. The bacteria of the genus Staphylococcus and Corynebacterium were resistant to penicillin (PEN), rifampicin (RIF), nitrofurantoin (NIT), erythromycin (ERI), and ciprofloxacin (CIP). The extract was used in vivo in the post-dipping of dairy cows, which reduced the microbiological load present in the mammary glands from 4.74 to 2.54 CFU, indicating its future use as an antiseptic.
牛乳腺炎是全球奶牛中最常见的疾病之一。在挤奶过程中使用防腐剂，如果使用得当，可以减少潜在致病微生物及其在牛群间的传播。几种药用植物具有防腐潜力，包括桉树（桉属植物）。因此，本研究的目的是评估巨桉克隆GG I144（EU）木醋液在体外和体内作为防腐剂的有效性；此外，还要评估其细胞毒性和抗菌耐药性。使用了15头奶牛，均为3至6岁的泌乳雌性奶牛，分为三组，每组5头。将木醋液置于动物乳头处28天，每7天收集细胞碎片。在UFERSA的兽医微生物学实验室（LAMIV），对样本进行处理，并在37℃下于含有平板计数琼脂（PCA）的培养皿中进行系列稀释。基于形态学改变和代谢活性验证细胞毒性。在与1%木醋提取物孵育的所有细胞中均未观察到形态学变化。体外数据表明其对金黄色葡萄球菌、无乳链球菌、沙门氏菌、大肠杆菌和铜绿假单胞菌具有抗菌活性。葡萄球菌属和棒状杆菌属细菌对青霉素（PEN）、利福平（RIF）、呋喃妥因（NIT）、红霉素（ERI）和环丙沙星（CIP）耐药。该提取物在奶牛乳头药浴中进行了体内应用，可将乳腺中的微生物负荷从4.74 CFU降至2.54 CFU，表明其未来可作为防腐剂使用。
