Shanghai Institute of Medical Imaging, Shanghai, China.
Department of Interventional Radiology, Zhongshan Hospital Fudan University, Shanghai, China.
J Cell Mol Med. 2021 Jan;25(2):1198-1206. doi: 10.1111/jcmm.16188. Epub 2020 Dec 20.
Calcium/calmodulin-dependent protein kinase (CAMKs) can control a wide range of cancer-related functions in multiple tumour types. Herein, we explore the expressions and clinical significances of calcium/calmodulin-dependent protein kinase 1 (CAMK1) in pancreatic cancer (PC). The expression of CAMK1 in PC was analysed by Gene Expression Profiling Interactive Analysis 2 (GEPIA 2) database and the Oncomine database. For further validation, the protein level of CAMK1 in PC tissues was also detected in the Human Protein Atlas (HPA) database and the tissue microarray (TMA)-based immunohistochemistry (IHC). GEPIA 2 and Kaplan-Meier Plotter (KM Plotter) databases were used to explore the prognostic significances of CAMK1 in overall survival (OS) and disease-free survival (DFS) of PC at mRNA level. The relationship between CAMK1 expression and the clinicopathological characteristics of PC was further explored. Additionally, the Search Tool for the Retrieval of Interacting Genes (STRING) database was used to analyse protein-protein interactions (PPI). We found CAMK1 was highly expressed in PC both in bioinformatics analyses and TMA-IHC results. The prognostic analyses from the public databases also showed consistent results with follow-up data. The PPI network suggested that CALM1, CALM3, CREB1, CALM2, SYN1, NOS3, ATF1, GAPDH, PPM1F and FBXL12 were important significant genes associated with CAMK1. Our finding revealed CAMK1 has prognostic value in PC patients, suggesting that CAMK1 may has a distinct role in PC patients and can be used as a candidate marker for investigating clinical prognosis of PC.
钙/钙调蛋白依赖性蛋白激酶（CAMKs）可以控制多种肿瘤类型中与癌症相关的多种功能。在此，我们探讨了钙/钙调蛋白依赖性蛋白激酶 1（CAMK1）在胰腺癌（PC）中的表达及其临床意义。通过基因表达谱分析交互分析 2（GEPIA 2）数据库和 Oncomine 数据库分析 PC 中 CAMK1 的表达。为了进一步验证，还在人类蛋白质图谱（HPA）数据库和基于组织微阵列（TMA）的免疫组织化学（IHC）中检测了 PC 组织中 CAMK1 的蛋白水平。GEPIA 2 和 Kaplan-Meier Plotter（KM Plotter）数据库用于探索 CAMK1 在 PC 患者总生存期（OS）和无病生存期（DFS）中的mRNA 水平的预后意义。进一步探讨了 CAMK1 表达与 PC 临床病理特征之间的关系。此外，还使用 Search Tool for the Retrieval of Interacting Genes（STRING）数据库分析了蛋白质-蛋白质相互作用（PPI）。我们发现，无论是在生物信息学分析还是 TMA-IHC 结果中，CAMK1 在 PC 中均呈高表达。来自公共数据库的预后分析也与随访数据显示出一致的结果。PPI 网络表明，CALM1、CALM3、CREB1、CALM2、SYN1、NOS3、ATF1、GAPDH、PPM1F 和 FBXL12 是与 CAMK1 相关的重要显著基因。我们的研究结果表明 CAMK1 在 PC 患者中具有预后价值，提示 CAMK1 在 PC 患者中可能具有独特的作用，并可作为研究 PC 临床预后的候选标志物。
