Mansouri Vahid, Arjmand Babak, Hamzeloo-Moghadam Maryam, Razzaghi Zahra, Ahmadzadeh Alireza, Ehsani Ardakani Mohammad Javad, Mohamoud Robati Reza
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2023;16(4):401-407. doi: 10.22037/ghfbb.v16i4.2859.
Due to weak diagnosis and treatment of pancreatic ductal adenocarcinoma (PDAC), detection of PDAC possible biomarkers in early stage is the main aim of this study.
PDAC is known as an exocrine cancer with a 5-year overall survival of 11%.
Gene expression profiles of early stage of PDAC tissue and normal tissue are downloaded from gene expression omnibus (GEO) and evaluated via GEO2R. The significant differentially expressed genes (DEGs) are investigated via protein-protein interaction (PPI) network analysis and gene ontology.
Among 104 DEGs, ALB, COL1A1, COL1A2, MMP1, POSTN, PLAU, and COL3A1 were pointed out as hub nodes. "Gelatin degradation by MMP1, 2, 3, 7, 8, 9, 12, 13" group of 52 biological terms were identified as the main affected terms.
In conclusion, ALB, MMP1, and COL1A1 genes were highlighted as possible biomarkers of early stage of PDAC. Dysfunction of extracellular matrix was identified as a main event in patients.
由于胰腺导管腺癌（PDAC）的诊断和治疗手段有限，本研究的主要目的是探寻PDAC早期可能的生物标志物。
PDAC是一种外分泌腺癌，其5年总生存率为11%。
从基因表达综合数据库（GEO）下载PDAC组织和正常组织早期的基因表达谱，并通过GEO2R进行评估。通过蛋白质-蛋白质相互作用（PPI）网络分析和基因本体论研究显著差异表达基因（DEG）。
在104个DEG中，白蛋白（ALB）、I型胶原蛋白α1（COL1A1）、I型胶原蛋白α2（COL1A2）、基质金属蛋白酶1（MMP1）、骨膜蛋白（POSTN）、纤溶酶原激活物（PLAU）和III型胶原蛋白α1（COL3A1）被指出是关键节点。52个生物学术语组成的“MMP1、2、3、7、8、9、12、13介导的明胶降解”组被确定为主要受影响的术语。
总之，ALB、MMP1和COL1A1基因被确定为PDAC早期可能的生物标志物。细胞外基质功能障碍被确定为患者的主要病变。
