State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730070, China.
Phytopathology. 2021 Aug;111(8):1449-1458. doi: 10.1094/PHYTO-03-20-0063-R. Epub 2021 Sep 16.
Xiaoyan6 (XY6) is a wheat () cultivar possessing nonrace-specific high-temperature seedling plant (HTSP) resistance against stripe rust, caused by f. sp. . Previously, we identified one particular gene, , for its involvement in the HTSP resistance. To elucidate the role of in the HTSP resistance, we cloned the full length of from XY6. The transcriptional expression of was rapidly upregulated (19.11-fold) under the normal-high-normal temperature treatment that induces the HTSP resistance. The expression level of in leaves was higher than that in the stems and roots. Quantification of the endogenous hormones in wheat leaves after f. sp. inoculation showed that 1-aminocyclopropane-1-carboxylic acid, salicylic acid (SA), and jasmonic acid were involved in the HTSP resistance. In addition, detection of hydrogen peroxide (HO) accumulation indicated that reactive oxygen species burst was also associated with the HTSP resistance. Two hours after exogenous HO treatment or 0.5 h after SA treatment, the expression level of was increased by 2.66 and 2.35 times, respectively. The subcellular localization of enhanced green fluorescent protein-TaRPS2 fusion protein was in the nuclei and plasma membranes. Virus-induced gene silencing of reduced the level of HTSP resistance in XY6. Compared with the nonsilenced leaves, the -silenced leaves had the reduction of necrotic cells but a greater number of uredinia. These results indicated that positively regulates the HTSP resistance of XY6 against f. sp. and is related to the SA and HO signaling pathways.
小偃 6 号（XY6）是一个小麦品种，具有针对条锈病的非专化性高温幼苗植株（HTSP）抗性，由 f. sp.. 引起。先前，我们鉴定了一个特定的基因 ，其参与 HTSP 抗性。为了阐明 在 HTSP 抗性中的作用，我们从 XY6 中克隆了 的全长。在诱导 HTSP 抗性的正常-高-正常温度处理下， 的转录表达迅速上调（19.11 倍）。 在叶片中的表达水平高于茎和根中的表达水平。接种 f. sp. 后小麦叶片内源性激素的定量分析表明，1-氨基环丙烷-1-羧酸、水杨酸（SA）和茉莉酸参与了 HTSP 抗性。此外，过氧化氢（HO）积累的检测表明，活性氧爆发也与 HTSP 抗性有关。外源 HO 处理后 2 小时或 SA 处理后 0.5 小时， 的表达水平分别增加了 2.66 倍和 2.35 倍。增强型绿色荧光蛋白-TaRPS2 融合蛋白的亚细胞定位在细胞核和质膜中。 的病毒诱导基因沉默降低了 XY6 对 f. sp. 的 HTSP 抗性水平。与未沉默的叶片相比， -沉默的叶片坏死细胞减少，但夏孢子堆数量增加。这些结果表明， 正向调节 XY6 对 f. sp. 的 HTSP 抗性，与 SA 和 HO 信号通路有关。
