Positively Regulates Wheat High-Temperature Seedling-Plant Resistance to f. sp. .

RPM1 is a CC-NBS-LRR protein that was first shown to be required for resistance to pv. in . Our previous study showed that gene in wheat was upregulated about six times following infection by f. sp. () under high temperature, compared with normal temperature. To study the function of in wheat high-temperature seedling-plant (HTSP) resistance to , the full length of was cloned, with three copies each located on chromosomes 1A, 1B, and 1D. Transient expression of the TaRPM1-GFP fusion protein in indicated that TaRPM1 localizes in the cytoplasm and nucleus. Profiling expression indicated that transcription was rapidly upregulated upon inoculation under high temperature. In addition, was induced by exogenous salicylic acid hormone application. Silencing in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to in terms of reduced number of necrotic cells and increased uredinial length, whereas no obvious phenotypic changes were observed in -silenced leaves under normal temperature. Related defense genes and were downregulated in -silenced plants under high temperature. We conclude that is involved in HTSP resistance to in XY 6.