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正向调控小麦对叶锈菌的高温幼苗-植株抗性。

Positively Regulates Wheat High-Temperature Seedling-Plant Resistance to f. sp. .

作者信息

Wang Jiahui, Tian Wei, Tao Fei, Wang Jingjing, Shang Hongsheng, Chen Xianming, Xu Xiangming, Hu Xiaoping

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, China.

Agricultural Research Service, United States Department of Agriculture and Department of Plant Pathology, Washington State University, Pullman, WA, United States.

出版信息

Front Plant Sci. 2020 Jan 15;10:1679. doi: 10.3389/fpls.2019.01679. eCollection 2019.

DOI:10.3389/fpls.2019.01679
PMID:32010164
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6974556/
Abstract

RPM1 is a CC-NBS-LRR protein that was first shown to be required for resistance to pv. in . Our previous study showed that gene in wheat was upregulated about six times following infection by f. sp. () under high temperature, compared with normal temperature. To study the function of in wheat high-temperature seedling-plant (HTSP) resistance to , the full length of was cloned, with three copies each located on chromosomes 1A, 1B, and 1D. Transient expression of the TaRPM1-GFP fusion protein in indicated that TaRPM1 localizes in the cytoplasm and nucleus. Profiling expression indicated that transcription was rapidly upregulated upon inoculation under high temperature. In addition, was induced by exogenous salicylic acid hormone application. Silencing in wheat cultivar Xiaoyan 6 (XY 6) resulted in reduced HTSP resistance to in terms of reduced number of necrotic cells and increased uredinial length, whereas no obvious phenotypic changes were observed in -silenced leaves under normal temperature. Related defense genes and were downregulated in -silenced plants under high temperature. We conclude that is involved in HTSP resistance to in XY 6.

摘要

RPM1是一种CC-NBS-LRR蛋白，最初被证明是小麦对叶锈菌（Puccinia triticina）抗性所必需的。我们之前的研究表明，与常温相比，小麦中的该基因在高温下被叶锈菌（Puccinia triticina Erikss.）感染后上调了约6倍。为了研究该基因在小麦高温幼苗-成株（HTSP）对叶锈菌抗性中的功能，克隆了该基因的全长，其三个拷贝分别位于1A、1B和1D染色体上。TaRPM1-GFP融合蛋白在烟草中的瞬时表达表明TaRPM1定位于细胞质和细胞核中。对该基因表达的分析表明，在高温下接种叶锈菌后，其转录迅速上调。此外，该基因受外源水杨酸激素诱导。在小麦品种小偃6号（XY 6）中沉默该基因导致HTSP对叶锈菌的抗性降低，表现为坏死细胞数量减少和夏孢子堆长度增加，而在常温下沉默该基因的叶片未观察到明显的表型变化。在高温下，沉默该基因的植株中相关防御基因PR1和PR2表达下调。我们得出结论，该基因参与了小偃6号对叶锈菌的HTSP抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/be92b0b5680e/fpls-10-01679-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/de6de028f9e2/fpls-10-01679-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/ffd20f9f7aa7/fpls-10-01679-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/e5c31e404b4f/fpls-10-01679-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/169ee1c49355/fpls-10-01679-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/5f594c7d51c7/fpls-10-01679-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/410dfa1a714b/fpls-10-01679-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/b57946db6949/fpls-10-01679-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/be92b0b5680e/fpls-10-01679-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/de6de028f9e2/fpls-10-01679-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/ffd20f9f7aa7/fpls-10-01679-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/e5c31e404b4f/fpls-10-01679-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/169ee1c49355/fpls-10-01679-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/5f594c7d51c7/fpls-10-01679-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/410dfa1a714b/fpls-10-01679-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/b57946db6949/fpls-10-01679-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d566/6974556/be92b0b5680e/fpls-10-01679-g008.jpg

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