Graduate Institute of Health Industry Technology, Research Center for Food and Cosmetic Safety, and Research Center for Chinese Herbal Medicine, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan City 33303, Taiwan.
Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Linkou, Guishan Dist., Taoyuan City 33303, Taiwan.
Mediators Inflamm. 2020 Dec 2;2020:1702935. doi: 10.1155/2020/1702935. eCollection 2020.
is a traditional folk herb used to improve inflammation and fever in Taiwan. Previous studies showed that extract could ameliorate lipopolysaccharide-induced acute lung injury in mice. The aim of this study was to investigate whether water (HZW) and ethyl acetate (HZE) extracts suppressed eosinophil infiltration and airway hyperresponsiveness (AHR) in asthmatic mice, and decreased the inflammatory response and oxidative stress in tracheal epithelial cells. Human tracheal epithelial cells (BEAS-2B cells) were pretreated with various doses of HZW or HZE (1 g/ml-10 g/ml), and cell inflammatory responses were induced with IL-4/TNF-. In addition, female BALB/c mice sensitized with ovalbumin (OVA), to induce asthma, were orally administered with HZW or HZE. The result demonstrated that HZW significantly inhibited the levels of proinflammatory cytokines, chemokines, and reactive oxygen species in activated BEAS-2B cells. HZW also decreased ICAM-1 expression and blocked monocytic cells from adhering to inflammatory BEAS-2B cells . Surprisingly, HZW was more effective than HZE in suppressing the inflammatory response in BEAS-2B cells. Our results demonstrated that HZW significantly decreased AHR and eosinophil infiltration, and reduced goblet cell hyperplasia in the lungs of asthmatic mice. HZW also inhibited oxidative stress and reduced the levels of Th2 cytokines in bronchoalveolar lavage fluid. Our findings suggest that HZW attenuated the pathological changes and inflammatory response of asthma by suppressing Th2 cytokine production in OVA-sensitized asthmatic mice.
是台湾地区一种传统民间草药，用于改善炎症和发热。先前的研究表明， 提取物可以改善脂多糖诱导的小鼠急性肺损伤。本研究旨在探讨 水（HZW）和乙酸乙酯（HZE）提取物是否抑制哮喘小鼠嗜酸性粒细胞浸润和气道高反应性（AHR），并降低气管上皮细胞的炎症反应和氧化应激。用不同剂量的 HZW 或 HZE（1 g/ml-10 g/ml）预处理人气管上皮细胞（BEAS-2B 细胞），并用 IL-4/TNF-诱导细胞炎症反应。此外，用卵清蛋白（OVA）致敏雌性 BALB/c 小鼠以诱导哮喘，并用 HZW 或 HZE 灌胃。结果表明，HZW 显著抑制了激活的 BEAS-2B 细胞中促炎细胞因子、趋化因子和活性氧的水平。HZW 还降低了 ICAM-1 的表达，并阻止单核细胞黏附于炎症性 BEAS-2B 细胞 。令人惊讶的是，HZW 在抑制 BEAS-2B 细胞炎症反应方面比 HZE 更有效。我们的研究结果表明，HZW 显著降低了哮喘小鼠的 AHR 和嗜酸性粒细胞浸润，并减少了肺部杯状细胞增生。HZW 还抑制氧化应激并降低支气管肺泡灌洗液中 Th2 细胞因子的水平。我们的研究结果表明，HZW 通过抑制 OVA 致敏哮喘小鼠 Th2 细胞因子的产生，减轻了哮喘的病理变化和炎症反应。
