Department of Nursing, Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, No. 261, Wenhua 1st Rd., Guishan Dist., Taoyuan City 33303, Taiwan; Division of Allergy, Asthma, and Rheumatology, Department of Pediatrics, Chang Gung Memorial Hospital, Guishan Dist., Taoyuan City 33303, Taiwan.
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, No. 155-1, Sec. 2, Li-Nung St., Peitou, Taipei, Taiwan.
J Ethnopharmacol. 2017 Mar 6;199:30-38. doi: 10.1016/j.jep.2017.01.043. Epub 2017 Jan 21.
Previous studies showed that Helminthostachys zeylanica (L.) Hook. could reduce inflammatory responses in macrophage and brain astrocytes.
In the present study, we evaluated whether an ethyl acetate extract (HZE) or a water extract (HZW) of H. zeylanica could reduce inflammatory responses in lung epithelial cells and ameliorate lipopolysaccharide (LPS)-induced acute lung injury in mice.
Human lung epithelial A549 cells were pre-treated with HZE or HZW (1-10μg/mL), then stimulated with LPS. BALB/c mice received oral HZW for 7 consecutive days, then an intratracheal instillation of LPS to induce lung injury.
HZW reduced chemokine and proinflammatory cytokine production in LPS-activated A549 cells. HZW also suppressed ICAM-1 expression and reduced the adherence of acute monocytic leukemia cells to inflammatory A549 cells. HZE had less efficacy than HZW in suppressing inflammatory responses in A549 cells. In vivo, HZW significantly suppressed neutrophil infiltration and reduced the TNF-α and IL-6 levels in bronchoalveolar lavage fluid and serum from LPS-treated mice. HZW also modulated superoxide dismutase activity, glutathione, and myeloperoxidase activity in lung tissues from LPS-treated mice. HZW decreased the phosphorylation of mitogen-activated protein kinase and nuclear factor kappa B, and promoted heme oxygenase-1 expression in inflamed lung tissue from LPS-treated mice.
Our findings suggested that HZW reduced lung injury in mice by reducing oxidative stress and inflammatory responses. HZW also reduced inflammatory responses in human lung epithelial cells.
先前的研究表明，肾茶（Helminthostachys zeylanica（L.）Hook.）可以减轻巨噬细胞和脑星形胶质细胞中的炎症反应。
本研究旨在评估肾茶的乙酸乙酯提取物（HZE）或水提取物（HZW）是否可以减轻肺上皮细胞中的炎症反应，并改善脂多糖（LPS）诱导的小鼠急性肺损伤。
用 HZE 或 HZW（1-10μg/mL）预处理人肺上皮 A549 细胞，然后用 LPS 刺激。BALB/c 小鼠连续 7 天口服 HZW，然后用 LPS 气管内滴注诱导肺损伤。
HZW 降低了 LPS 激活的 A549 细胞中趋化因子和促炎细胞因子的产生。HZW 还抑制了 ICAM-1 的表达，并减少了急性单核细胞白血病细胞对炎症 A549 细胞的黏附。HZE 在抑制 A549 细胞中的炎症反应方面不如 HZW 有效。在体内，HZW 显著抑制中性粒细胞浸润，并降低 LPS 处理小鼠支气管肺泡灌洗液和血清中的 TNF-α和 IL-6 水平。HZW 还调节了 LPS 处理小鼠肺组织中的超氧化物歧化酶活性、谷胱甘肽和髓过氧化物酶活性。HZW 降低了 LPS 处理小鼠炎症肺组织中丝裂原活化蛋白激酶和核因子 kappa B 的磷酸化，并促进血红素加氧酶-1 的表达。
我们的研究结果表明，HZW 通过降低氧化应激和炎症反应减轻了小鼠的肺损伤。HZW 还降低了人肺上皮细胞中的炎症反应。
