Zhu Shenghu, Guan Linshu, Tan Xuemei, Li Guoquan, Sun Changjie, Gao Meng, Zhang Bao, Xu Lina
Jiangsu Hengshun Vinegar Industry Co., Ltd., Zhenjiang, China.
College of Pharmacy, Dalian Medical University, Dalian, China.
Front Pharmacol. 2020 Dec 4;11:585582. doi: 10.3389/fphar.2020.585582. eCollection 2020.
Aromatic vinegar with abundant bioactive components can be used as a food additive to assist the treatment of various diseases. However, its effect on non-alcoholic fatty liver disease (NAFLD) is still unknown. The purpose of this study was to investigate the mechanism of Hengshun aromatic vinegar in preventing NAFLD and . Aromatic vinegar treatment was applied to rats fed with a high-fat diet (HFD) and HepG2 cells challenged with palmitic acid (PA). Our results showed that aromatic vinegar markedly improved cell viabilities and attenuated cell damage . The levels of TC, TG, FFA, AST, ALT, and malondialdehyde (MDA) in HFD-induced rats were significantly decreased by aromatic vinegar. Mechanism investigation revealed that aromatic vinegar markedly up-regulated the level of silent information regulator of transcription 1 (Sirt1), and thereby inhibited inflammation of the pathway through down-regulating the expressions of high mobility group box 1, toll-likereceptor-4, nuclear transcription factor-κB, tumor necrosis factor receptor-associated factor-6, and inflammatory factors. Aromatic vinegar simultaneously increased the expression of farnesoid X receptor and suppressed expressions of lipogenesis related proteins, including fatty acid synthase, acetyl-CoA carboxylase-1, sterol regulatory element binding transcription factor 1, and stearoyl-CoA desaturase-1. These results were further validated by knockdown of Sirt1 using siRNAs silencing . In conclusion, Hengshun aromatic vinegar showed protective effects against NAFLD by enhancing the activity of SIRT1 and thereby inhibiting lipogenesis and inflammation pathways, which is expected to become a new assistant strategy for NAFLD therapy in the future.
含有丰富生物活性成分的香醋可作为食品添加剂用于辅助治疗各种疾病。然而，其对非酒精性脂肪性肝病（NAFLD）的影响仍不清楚。本研究的目的是探讨恒顺香醋预防NAFLD的机制。将香醋处理应用于高脂饮食（HFD）喂养的大鼠和用棕榈酸（PA）刺激的HepG2细胞。我们的结果表明，香醋显著提高了细胞活力并减轻了细胞损伤。香醋显著降低了HFD诱导大鼠的总胆固醇（TC）、甘油三酯（TG）、游离脂肪酸（FFA）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和丙二醛（MDA）水平。机制研究表明，香醋显著上调了沉默信息调节因子1（Sirt1）的水平，从而通过下调高迁移率族蛋白B1、Toll样受体4、核转录因子κB、肿瘤坏死因子受体相关因子6和炎症因子的表达来抑制该通路的炎症。香醋同时增加了法尼酯X受体的表达并抑制了脂肪生成相关蛋白的表达，包括脂肪酸合酶、乙酰辅酶A羧化酶1、固醇调节元件结合转录因子1和硬脂酰辅酶A去饱和酶1。使用小干扰RNA（siRNAs）沉默Sirt1进一步验证了这些结果。总之，恒顺香醋通过增强SIRT1的活性从而抑制脂肪生成和炎症通路，对NAFLD具有保护作用，有望成为未来NAFLD治疗的一种新的辅助策略。
