Al-Saman Mahmoud A, Doleib Nada M, Ibrahim Mohamed R, Nasr Mohamed Y, Tayel Ahmed A, Hamouda Ragaa A
Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt.
Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Jeddah, Saudi Arabia.
PeerJ. 2020 Dec 1;8:e10366. doi: 10.7717/peerj.10366. eCollection 2020.
is blue-green algae that grows mainly in tropical and subtropical lakes and is commonly used due to its nutritional features including high concentrations of protein, vitamins, mineral salts, carotenoids and antioxidants. This study aimed to investigate the anti-hypercholesterolemic potential of aqueous extract of cultivated in different colored flasks under artificial illumination; in vitro and in the diet induced hypercholesterolemic Swiss albino mice.
was cultivated in red, blue, green and colorless Erlenmeyer flasks containing Zarrouk's medium under aerobic conditions, with incessant illumination by artificial cool white fluorescent with light intensity of 2500 lux (35 µmol photon m s). Chlorophyll a and total carotenoid contents were estimated using colorimetric methods, fatty acids composition was determined by GC-Mass, in vitro and in vivo anti-cholesterol assays were used in assessing the anti-hypercholesterolemia potential of obtained cells.
The results showed that the highest cell dry weight, chlorophyl a, and carotenoid of were observed in colorless flasks and that the lowest values were recorded with the green colored flasks. Also, the hot water extract of obtained from colorless flasks at a concentration of 15 mg/mL after 60 min of incubation exhibited the greatest reduction of cholesterol level. Gas chromatography-mass spectrometry analysis of methanolic extract showed 15 bioactive compounds were identified and grouped according to their chemical structures. An experimental model of hypercholesterolemic mice had been examined for impact of individually and combined with atorvastatin drug. All groups resulted in a remarkable decrease in plasma total cholesterol, triglycerides and low density lipoprotein; and increase in high density lipoprotein.
The present study concluded that the hot aqueous extract of developed in colorless flasks is recommended as a natural source for bioactive compounds, with anti-cholesterol and antioxidant potentialities.
[藻名未给出]是一种主要生长在热带和亚热带湖泊中的蓝绿藻，因其营养特性，包括高浓度的蛋白质、维生素、矿物盐、类胡萝卜素和抗氧化剂，而被广泛使用。本研究旨在调查在人工光照下于不同颜色烧瓶中培养的[藻名未给出]水提取物的抗高胆固醇潜力；在体外以及对饮食诱导的高胆固醇瑞士白化小鼠进行研究。
[藻名未给出]在含有扎鲁克培养基的红色、蓝色、绿色和无色锥形烧瓶中在有氧条件下培养，用人工冷白色荧光持续光照，光强度为2500勒克斯（35微摩尔光子/平方米·秒）。使用比色法估计叶绿素a和总类胡萝卜素含量，通过气相色谱 - 质谱法测定脂肪酸组成，使用体外和体内抗胆固醇测定法评估所得[藻名未给出]细胞的抗高胆固醇血症潜力。
结果表明，在无色烧瓶中观察到[藻名未给出]的细胞干重、叶绿素a和类胡萝卜素最高，而在绿色烧瓶中记录到最低值。此外，在无色烧瓶中培养60分钟后获得的浓度为15毫克/毫升的[藻名未给出]热水提取物显示出最大程度的胆固醇水平降低。[藻名未给出]甲醇提取物的气相色谱 - 质谱分析表明鉴定出15种生物活性化合物，并根据其化学结构进行了分类。对高胆固醇小鼠的实验模型单独以及与阿托伐他汀药物联合检查了[藻名未给出]的影响。所有[藻名未给出]组均导致血浆总胆固醇、甘油三酯和低密度脂蛋白显著降低；高密度脂蛋白增加。
本研究得出结论，在无色烧瓶中培养的[藻名未给出]热含水提取物被推荐作为生物活性化合物的天然来源，具有抗胆固醇和抗氧化潜力。
