Ama Moor Vicky Jocelyne, Nya Biapa Prosper Cabral, Nono Njinkio Borgia Legrand, Moukette Moukette Bruno, Sando Zacharie, Kenfack Cyril, Ateba Baruch, Ngo Matip Marthe Elise, Pieme Constant Anatole, Ngogang Jeanne
1Department Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences - University of Yaounde 1, Yaounde, Cameroon.
2Department of Biochemistry, Faculty of Sciences, University of Dschang, Dschang, Cameroon.
BMC Nutr. 2017 Mar 14;3:25. doi: 10.1186/s40795-017-0146-2. eCollection 2017.
produced in Nomayos (Cameroon) is used as a dietary supplement. is known as a neutraceutical with many beneficial effects on humans like lipid-lowering action. This study aims to investigate the mechanism of hypolipidemic action of aqueous extract of () through the toxicological studies.
In this study, we included two month old Wistar rats, weighing between 180 and 200 g. Aqueous was extracted and prepared using standard methods. The rats received a supplementation of at 5000 mg/Kg of body weight as single dose in acute toxicity whereas different doses (250, 500, 1000 mg / kg body weight) were administered in subacute toxicity compared to control. Acute and subacute toxicities were determined according to the guidelines 420 (14 days) and 407 (28 days) of the Organization for Economic Cooperation and Development (OECD) respectively. Biochemical parameters such as urea, creatinine, total and direct bilirubin, lipid profile and transaminases; and histopathological analysis of the liver and kidneys were used to evaluate the toxicity of S. platensis on these Wistar rats. Plasmatic hydroxymethyl glutaryl coenzyme A reductase (HMG CoA reductase) and lecithine cholesterol acyl transferase (LCAT) were performed to explain the lipid-lowering action of . Histopathological analysis of the liver and kidneys was performed.
Our results show a decrease in total cholesterol for male rats (from 84 to 74 mg/dl) when the dose of increased; this reduction of the total cholesterol level in male rats was significant at 500 mg/kg. There was also a significant inhibition of HMG CoA reductase in a dose dependent manner between 25 and 84.5 fold compared to the control in both male and female groups. At the dose of 250 mg/kg bw, the level of LCAT was higher compared with other groups and control, but the difference was not statistically significant. A slight inflammation in the liver and the mesangial hyperplasia of the renal glomeruli was revealed by the histopathological investigation in subacute toxicity
from Cameroon appears to have little toxic effects and may demonstrate hypolipidemic activity through the activation of LCAT.
产自诺马约斯（喀麦隆）的[某种物质，原文未明确]被用作膳食补充剂。它作为一种营养保健品，对人体有许多有益作用，如降血脂作用。本研究旨在通过毒理学研究探讨[某种物质，原文未明确]水提取物的降血脂作用机制。
在本研究中，我们选用了两个月大、体重在180至200克之间的Wistar大鼠。采用标准方法提取并制备[某种物质，原文未明确]水提取物。在急性毒性实验中，大鼠以5000毫克/千克体重的剂量单次接受[某种物质，原文未明确]补充；在亚急性毒性实验中，与对照组相比，给予不同剂量（250、500、1000毫克/千克体重）的[某种物质，原文未明确]。急性和亚急性毒性分别根据经济合作与发展组织（OECD）的420号指南（14天）和407号指南（28天）进行测定。使用尿素、肌酐、总胆红素和直接胆红素、血脂谱及转氨酶等生化参数，以及肝脏和肾脏的组织病理学分析来评估[某种物质，原文未明确]对这些Wistar大鼠的毒性。检测血浆羟甲基戊二酰辅酶A还原酶（HMG CoA还原酶）和卵磷脂胆固醇酰基转移酶（LCAT）以解释[某种物质，原文未明确]的降血脂作用。进行肝脏和肾脏的组织病理学分析。
我们的结果显示，随着[某种物质，原文未明确]剂量增加，雄性大鼠的总胆固醇水平降低（从84毫克/分升降至74毫克/分升）；当剂量为500毫克/千克时，雄性大鼠总胆固醇水平的降低具有显著性。在雄性和雌性组中，与对照组相比，HMG CoA还原酶也以剂量依赖方式受到显著抑制，抑制倍数在25至84.5倍之间。在250毫克/千克体重的剂量下，LCAT水平高于其他组和对照组，但差异无统计学意义。亚急性毒性的组织病理学研究显示肝脏有轻微炎症，肾小球系膜增生。
来自喀麦隆的[某种物质，原文未明确]似乎毒性很小，可能通过激活LCAT表现出降血脂活性。
