用非极性树脂XAD - 2吸收法浓缩尿液中的诱变剂：吸烟者的尿液具有致突变性。
Concentration of mutagens from urine by absorption with the nonpolar resin XAD-2: cigarette smokers have mutagenic urine.
作者信息
Yamasaki E, Ames B N
出版信息
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3555-9. doi: 10.1073/pnas.74.8.3555.
Abstract
A method is described for concentrating mutagens/carcinogens from human urine about 200-fold for subsequent assay in the Salmonella/mammalian microsome mutagenicity test. The method is also applicable for other aqueous liquids and for other in vitro tests for mutagens/carcinogens. The urine (up to 500 ml) is put through a column with a 1.5-cm3 bed volume of XAD-2 (styrene-divinylbenzene polymer) and the adsorbed material is then eluted with a few milliliters of acetone. The acetone is taken to dryness and the residue is dissolved in dimethyl sulfoxide. This is the urine concentrate that is assayed for mutagenicity. Various mutagens/carcinogens have been added to human urine and the recoveries have been measured after adsorption on XAD-2, XAD-4, and Tenax GC (diphenyl-p-phenylene oxide polymer). We propose that this method be used in monitoring the urine of human populations and of experimental animals in toxicological studies. It is shown with this procedure that cigarette smokers have mutagenic urine while nonsmokers do not.
摘要
本文描述了一种将人尿中的诱变剂/致癌物浓缩约200倍的方法，以便随后在沙门氏菌/哺乳动物微粒体诱变试验中进行检测。该方法也适用于其他水性液体以及其他诱变剂/致癌物的体外试验。将尿液（最多500毫升）通过装有1.5立方厘米床体积XAD - 2（苯乙烯 - 二乙烯基苯聚合物）的柱子，然后用几毫升丙酮洗脱吸附的物质。将丙酮蒸干，残余物溶解在二甲基亚砜中。这就是用于诱变检测的尿浓缩物。已将各种诱变剂/致癌物添加到人尿中，并测定了它们在XAD - 2、XAD - 4和Tenax GC（二苯基 - 对 - 苯撑氧化物聚合物）上吸附后的回收率。我们建议在毒理学研究中使用该方法监测人群和实验动物的尿液。通过该程序表明，吸烟者的尿液具有诱变性，而非吸烟者则没有。
相似文献
1
Concentration of mutagens from urine by absorption with the nonpolar resin XAD-2: cigarette smokers have mutagenic urine.用非极性树脂XAD - 2吸收法浓缩尿液中的诱变剂：吸烟者的尿液具有致突变性。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3555-9. doi: 10.1073/pnas.74.8.3555.
2
Isolation of frameshift mutagens from smokers' urine: experiences with three concentration methods.
Carcinogenesis. 1985 May;6(5):783-8. doi: 10.1093/carcin/6.5.783.
3
Detection of mutagens in human urine by means of XAD-2 adsorption-desorption techniques and bacterial mutagenesis.
Pharmacol Res Commun. 1983 Sep;15(8):775-82. doi: 10.1016/s0031-6989(83)80006-x.
4
The use of rat urine extracted on XAD-2 resin and assayed in a microsuspension-modified Ames test as an in vivo indicator of genotoxic exposure.
Mutat Res. 1988 Oct;206(2):141-8. doi: 10.1016/0165-1218(88)90153-x.
5
Analysis of mutagenic activity in human urine after concentration on different resins and high-performance liquid chromatography.
Toxicol Lett. 1989 Feb;45(2-3):241-9. doi: 10.1016/0378-4274(89)90015-5.
6
The urine mutagenicity assay system. Studies related to recovery, storage and concentration procedures.
Scand J Work Environ Health. 1985 Feb;11(1):45-50. doi: 10.5271/sjweh.2254.
7
Urinary mutagenicity assays: a problem arising from the presence of histidine associated growth factors in XAD-2 prepared urine concentrates, with particular relevance to assays carried out using the bacterial fluctuation test.
Carcinogenesis. 1983 Nov;4(11):1471-6. doi: 10.1093/carcin/4.11.1471.
8
Mutagenicity of urine from young male smokers and nonsmokers.
Int Arch Occup Environ Health. 1987;59(1):1-9. doi: 10.1007/BF00377673.
9
Analysis of urinary mutagens produced by cigarette-smoking baboons.
Mutat Res. 1983 Sep;118(4):241-56. doi: 10.1016/0165-1218(83)90208-2.
10
Limitations of the salmonella/mammalian microsome assay (Ames test) to determine occupational exposure to cytostatic drugs.
Eur J Cancer Clin Oncol. 1986 May;22(5):567-75. doi: 10.1016/0277-5379(86)90045-3.
引用本文的文献
1
Urinary mutagenicity and bladder cancer risk in northern New England.新英格兰北部的尿液致突变性与膀胱癌风险。
Environ Mol Mutagen. 2024 Jan-Feb;65(1-2):47-54. doi: 10.1002/em.22588. Epub 2024 Mar 11.
2
History of the Relationship Between Smoking and Bladder Cancer: A Public Health Perspective.吸烟与膀胱癌关系的历史：公共卫生视角
Urology. 2023 Jan;171:6-10. doi: 10.1016/j.urology.2022.08.007. Epub 2022 Aug 14.
3
Risk factors for recurrence of colorectal conventional adenoma and serrated polyp.结直肠传统腺瘤和锯齿状息肉复发的危险因素。
Gastroenterol Rep (Oxf). 2021 Sep 16;10:goab038. doi: 10.1093/gastro/goab038. eCollection 2022.
4
Synergistic Effects of Genetic Variants of Glucose Homeostasis and Lifelong Exposures to Cigarette Smoking, Female Hormones, and Dietary Fat Intake on Primary Colorectal Cancer Development in African and Hispanic/Latino American Women.葡萄糖稳态基因变异与终生接触吸烟、女性激素及膳食脂肪摄入对非洲裔和西班牙裔/拉丁裔美国女性原发性结直肠癌发生的协同作用。
Front Oncol. 2021 Oct 7;11:760243. doi: 10.3389/fonc.2021.760243. eCollection 2021.
5
Genetic Signatures of Glucose Homeostasis: Synergistic Interplay With Long-Term Exposure to Cigarette Smoking in Development of Primary Colorectal Cancer Among African American Women.葡萄糖稳态的遗传特征：长期暴露于吸烟与非裔美国女性原发性结直肠癌发生的协同作用。
Clin Transl Gastroenterol. 2021 Oct 5;12(10):e00412. doi: 10.14309/ctg.0000000000000412.
6
Elevated urinary mutagenicity among those exposed to bituminous coal combustion emissions or diesel engine exhaust.在接触沥青燃烧排放物或柴油发动机废气的人群中，尿液诱变活性升高。
Environ Mol Mutagen. 2021 Oct;62(8):458-470. doi: 10.1002/em.22455. Epub 2021 Aug 16.
7
Genetically determined elevated C-reactive protein associated with primary colorectal cancer risk: Mendelian randomization with lifestyle interactions.遗传决定的C反应蛋白升高与原发性结直肠癌风险相关：与生活方式相互作用的孟德尔随机化研究
Am J Cancer Res. 2021 Apr 15;11(4):1733-1753. eCollection 2021.
8
Post Genome-Wide Gene-Environment Interaction Study Using Random Survival Forest: Insulin Resistance, Lifestyle Factors, and Colorectal Cancer Risk.基于随机生存森林的全基因组基因-环境交互作用研究：胰岛素抵抗、生活方式因素与结直肠癌风险。
Cancer Prev Res (Phila). 2019 Dec;12(12):877-890. doi: 10.1158/1940-6207.CAPR-19-0278. Epub 2019 Sep 25.
9
Risk Factors for Recurrent Colorectal Polyps.结直肠息肉复发的危险因素。
Gut Liver. 2020 Jul 15;14(4):399-411. doi: 10.5009/gnl19097.
10
TNBC vs. Non-TNBC: A Five-Year Retrospective Review of Differences in Mean Age, Family History, Smoking History and Stage at Diagnosis at an Inner City University Program.三阴性乳腺癌与非三阴性乳腺癌：对一所市中心大学项目中平均年龄、家族史、吸烟史及诊断时分期差异的五年回顾性研究
World J Oncol. 2013 Dec;4(6):241-247. doi: 10.4021/wjon738w. Epub 2014 Jan 16.
本文引用的文献
1
Smoking and cancer of the lower urinary tract.吸烟与下尿路癌症
N Engl J Med. 1971 Jan 21;284(3):129-34. doi: 10.1056/NEJM197101212840304.
2
The analytical concentration of traces of dissolved organic materials from sea water with Amberlite XAD-1 resin.
Anal Chim Acta. 1969 Jul;46(2):307-9. doi: 10.1016/S0003-2670(01)95630-2.
3
Identification and estimation of neutral organic contaminants in potable water.
Anal Chem. 1972 Jan;44(1):139-42. doi: 10.1021/ac60309a005.
4
Oxidative metabolism of nicotine by cigarette smokers with cancer of the urinary bladder.
J Natl Cancer Inst. 1974 May;52(5):1421-4. doi: 10.1093/jnci/52.5.1421.
5
Studies on the use of XAD-2 resin for detection of abused drugs in urine.
Clin Chem. 1974 Feb;20(2):177-83.
6
Polychlorobiphenyls in North Atlantic ocean water.
Science. 1973 May 11;180(4086):643-4. doi: 10.1126/science.180.4086.643.
7
Detection of mutagenic activity in cigarette smoke condensates.香烟烟雾冷凝物中诱变活性的检测。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4159-63. doi: 10.1073/pnas.71.10.4159.
8
A simple method for the detection of mutagens in urine: studies with the carcinogen 2-acetylaminofluorene.一种检测尿液中诱变剂的简单方法：用致癌物2-乙酰氨基芴进行的研究。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):737-41. doi: 10.1073/pnas.71.3.737.
9
Use of macroreticular resins in the analysis of water for trace organic contaminants.大孔网状树脂在分析水中痕量有机污染物中的应用。
J Chromatogr. 1974 Nov 6;99(0):745-62. doi: 10.1016/s0021-9673(00)90900-2.
10
Routine identification of drugs of abuse in human urine. II. Development and application of the XAD-2 resin column method.
J Chromatogr. 1971 Dec 23;63(2):289-301. doi: 10.1016/s0021-9673(01)85641-7.
Zotero 插件