血必净注射液对接受辐照的小鼠肠损伤的保护作用。
The protective effects of Xuebijing injection on intestinal injuries of mice exposed to irradiation.
机构信息
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, China.
Key Laboratory of Human Disease Comparative Medicine, Ministry of Health, Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, National Human Diseases Animal Model Resource Center, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing, China.
出版信息
Animal Model Exp Med. 2022 Dec;5(6):565-574. doi: 10.1002/ame2.12285. Epub 2022 Nov 14.
BACKGROUND
Gastrointestinal (GI) injury is one of the most common side effects of radiotherapy. However, there is no ideal therapy method except for symptomatic treatment in the clinic. Xuebijing (XBJ) is a traditional Chinese medicine, used to treat sepsis by injection. In this study, the protective effects of XBJ on radiation-induced intestinal injury (RIII) and its mechanism were explored.
METHODS
The effect of XBJ on survival of irradiated C57BL/6 mice was monitored. Histological changes including the number of crypts and the length of villi were evaluated by H&E. The expression of Lgr5 intestinal stem cells (ISCs), Ki67 cells, villin and lysozymes were examined by immunohistochemistry. The expression of cytokines in the intestinal crypt was detected by RT-PCR. DNA damage and apoptosis rates in the small intestine were also evaluated by immunofluorescence.
RESULTS
In the present study, XBJ improved the survival rate of the mice after 8.0 and 9.0 Gy total body irradiation (TBI). XBJ attenuated structural damage of the small intestine, maintained regenerative ability and promoted proliferation and differentiation of crypt cells, decreased apoptosis rate and reduced DNA damage in the intestine. Elevation of IL-6 and TNF-α was limited, but IL-1, TNF-𝛽 and IL-10 levels were increased in XBJ-treated group after irradiation. The expression of Bax and p53 were decreased after XBJ treatment.
CONCLUSIONS
Taken together, XBJ provides a protective effect on RIII by inhibiting inflammation and blocking p53-related apoptosis pathway.
背景
胃肠道（GI）损伤是放射治疗最常见的副作用之一。然而，临床上除了对症治疗外，没有理想的治疗方法。血必净（XBJ）是一种中药，用于注射治疗脓毒症。在这项研究中，探讨了 XBJ 对放射性肠损伤（RIII）的保护作用及其机制。
方法
监测 XBJ 对接受放射照射的 C57BL/6 小鼠的生存影响。通过 H&E 评估隐窝数量和绒毛长度等组织学变化。通过免疫组织化学检测 Lgr5 肠干细胞（ISCs）、Ki67 细胞、绒毛蛋白和溶菌酶的表达。通过 RT-PCR 检测肠隐窝中的细胞因子表达。通过免疫荧光评估小肠中的 DNA 损伤和细胞凋亡率。
结果
在本研究中，XBJ 提高了接受 8.0 和 9.0Gy 全身照射（TBI）的小鼠的存活率。XBJ 减轻了小肠的结构损伤，维持了再生能力，促进了隐窝细胞的增殖和分化，降低了肠细胞的凋亡率和 DNA 损伤。照射后，IL-6 和 TNF-α 的升高受到限制，但 XBJ 处理组中 IL-1、TNF-𝛽 和 IL-10 水平升高。XBJ 处理后 Bax 和 p53 的表达减少。
结论
综上所述，XBJ 通过抑制炎症和阻断 p53 相关的细胞凋亡途径对 RIII 提供保护作用。
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