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通过直接重复的插入序列2元件处的重组切除F质粒序列:recA的参与

Excision of F plasmid sequences by recombination at directly repeated insertion sequence 2 elements: involvement of recA.

作者信息

Deonier R C, Mirels L

出版信息

Proc Natl Acad Sci U S A. 1977 Sep;74(9):3965-9. doi: 10.1073/pnas.74.9.3965.

Abstract

The DNA of the F plasmid is joined to bacterial DNA sequences in the F' ORF203 by directly repeated insertion sequence 2 (IS2) elements. The rate of excision of the F plasmid form this F' (presumably by recombination at the directly repeated IS2s) has been estimated in both recA+ and recA- strains. Normal F is produced in the recA+ strain, but is not detected in recA-. The autonomous plasmids produced in the recA- background were F's having deletions. F excision in this particular recA+ case is specific in the sense that the directly repeated IS2s appear to be more active in recombination than similarly disposed IS3 direct repetitions in this F'.

摘要

F质粒的DNA通过直接重复的插入序列2(IS2)元件与F' ORF203中的细菌DNA序列相连。在recA +和recA-菌株中均已估计了F质粒从该F'切除的速率(推测是通过直接重复的IS2处的重组)。在recA +菌株中产生正常F,但在recA-中未检测到。在recA-背景中产生的自主质粒是具有缺失的F'。在这种特定的recA +情况下,F切除具有特异性,因为直接重复的IS2在重组中似乎比该F'中类似排列的IS3直接重复更活跃。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fd44/431803/0e638448b443/pnas00031-0350-a.jpg

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