de Vries P, Van Binnendijk R S, Van der Marel P, Beuvery E C, Van Wezel A L, Voorma H O, Uytedehaag F G, Osterhaus A D
National Institute of Public Health and Environmental Hygiene, Bilthoven, The Netherlands.
Viral Immunol. 1987 Spring;1(1):25-34. doi: 10.1089/vim.1987.1.25.
A neutralizing monoclonal antibody (C26-15) against the haemagglutinin (H protein) of measles virus was generated which caused cell-cell fusion inhibition in cultures of measles virus-infected cells. It was shown that this phenomenon coincided with a down-regulation of the expression of both the H protein and the fusion (F) protein. We also showed cell-cell fusion inhibition with a polyclonal rabbit serum directed against Tween-ether inactivated measles virus, which did not contain biologically active antibodies against the F protein. Cell-cell fusion inhibition caused by anti-H antibodies is distinct from cell-cell fusion inhibition induced by a direct interaction of anti-F antibody with the F protein in the membrane of infected cells. Since both mechanisms may also be involved in the in vivo situation, the exclusive role for the generation of anti-F antibody to prevent virus spread by cell-cell fusion in vivo is questioned. It is speculated that the observed down-regulation of both glycoproteins may lead to a less efficient killing of infected cells by cytotoxic T-lymphocytes, which may constitute an alternative explanation for the insufficient protection after vaccination with an inactivated measles vaccine.
制备了一种针对麻疹病毒血凝素（H蛋白）的中和单克隆抗体（C26 - 15），该抗体在麻疹病毒感染细胞的培养物中可引起细胞 - 细胞融合抑制。结果表明，这一现象与H蛋白和融合（F）蛋白表达的下调同时出现。我们还使用针对吐温 - 乙醚灭活麻疹病毒的兔多克隆血清显示了细胞 - 细胞融合抑制，该血清不含针对F蛋白的生物活性抗体。抗H抗体引起的细胞 - 细胞融合抑制不同于抗F抗体与感染细胞膜上的F蛋白直接相互作用诱导的细胞 - 细胞融合抑制。由于这两种机制在体内情况中可能都起作用，因此抗F抗体在体内通过细胞 - 细胞融合预防病毒传播的唯一作用受到质疑。据推测，观察到的两种糖蛋白的下调可能导致细胞毒性T淋巴细胞对感染细胞的杀伤效率降低，这可能是接种灭活麻疹疫苗后保护不足的另一种解释。
