ter Meulen V, Löffler S, Carter M J, Stephenson J R
J Gen Virol. 1981 Dec;57(Pt 2):357-64. doi: 10.1099/0022-1317-57-2-357.
Hybrid cells secreting monoclonal antibodies directed against the haemagglutinin (H) protein of measles virus (Edmonston) were produced by fusion of mouse myeloma cells with spleen cells derived from immunized mice. Measles antibodies secreted by these cells were tested for their ability to react with measles virus in immunoprecipitation experiments and assays of binding, neutralization, haemagglutination inhibition and haemolysin inhibition. On this basis 21 out of 75 hybridomas could be defined and divided into five functional groups with different properties. However, when tested against other measles virus strains, including those isolated from subacute sclerosing panencephalitis (SSPE) patients, normalized radioimmunoassay (RIA) binding titres showed that the extent to which a given antibody bound could vary greatly with the virus strain examined. Moreover, the biological actions within a group were found to be very heterogeneous, even when high antibody binding titres were observed. These results suggest that different measles virus strains, which are not distinguishable by polyvalent sera, do in fact possess antigenic differences. Furthermore, the functional significance of a given virus epitope may vary from strain to strain. Hybridoma antibodies were also used to demonstrate the occurrence of antigenic changes within the H polypeptide of SSPE virus during the course of non-productive, persistent infection in vitro.
通过将小鼠骨髓瘤细胞与来自免疫小鼠的脾细胞融合，产生了分泌针对麻疹病毒（埃德蒙斯顿株）血凝素（H）蛋白的单克隆抗体的杂交细胞。对这些细胞分泌的麻疹抗体在免疫沉淀实验以及结合、中和、血凝抑制和溶血素抑制试验中与麻疹病毒反应的能力进行了检测。在此基础上，75个杂交瘤中的21个可被鉴定并分为具有不同特性的五个功能组。然而，当用包括从亚急性硬化性全脑炎（SSPE）患者分离出的毒株在内的其他麻疹病毒株进行检测时，标准化放射免疫分析（RIA）结合滴度显示，给定抗体的结合程度会因所检测的病毒株而有很大差异。此外，即使观察到高抗体结合滴度，一组内的生物学活性也被发现非常不均一。这些结果表明，不能通过多价血清区分的不同麻疹病毒株实际上存在抗原差异。此外，给定病毒表位的功能意义可能因毒株而异。杂交瘤抗体还被用于证明在体外非生产性持续感染过程中，SSPE病毒H多肽内发生了抗原变化。
