Department of Biotechnology, Jamia Hamdard, New Delhi, India.
Department of Chemistry, Indian Institute of Technology Delhi, New Delhi, India.
Microbiol Immunol. 2021 Aug;65(8):290-301. doi: 10.1111/1348-0421.12869. Epub 2021 Jul 12.
Chikungunya virus (CHIKV), an arthropod-borne Alphavirus is responsible for chikungunya disease. Arthralgia and arthritis are the major symptom. Some patients recover early while others for a very long time. This study provides, epidemiology and molecular characterization of three whole-genome sequences of CHIKV and assessed phylogenetic analysis, physiological properties, antigenicity, and B-cell epitope prediction by in silico. We report the clinical epidemiology of 325 suspected patients. Of these, 118 (36.30%) were confirmed CHIKV positive by either PCR or ELISA. Clinical analysis showed joint pain, joint swelling and headache were frequent and significant features. Phylogenie analysis showed the currently circulating strain is in close clustring to Africa, Uganda, and Singapore CHIKV strains. Molecular characterization by WGS was done. Thirty eight amino acid changes in the nonstructural proteins were found with respect to the S27 (ECSA) strain. Of these five located in nsP2. Similarly, 34 amino acid changes in structural proteins were observed. The major change was notice; in E3 protein hydropathicity -0.281 to -0.362, in E2 isoelectric point (pI) 8.24 to 8.37, instability index 66.08 to 71.062, aliphatic index varied from 74.69 to 68.59 and E3 75.79 to 70.05. In nsP1 protein pI varies from 6.62 to 8.04, while no other change was observed in structural and nonstructural protein. The linear B-cell epitopes, position, and number varied with the mutation. The molecular characterizations of WGS demonstrate the observation of protein, antigenicity with respect to the mutation.
基孔肯雅病毒（CHIKV）是一种节肢动物传播的阿尔法病毒，可引起基孔肯雅热。关节痛和关节炎是主要症状。有些患者恢复得较早，而有些患者则恢复得非常缓慢。本研究提供了 3 株基孔肯雅病毒的全基因组序列的流行病学和分子特征，并通过计算机模拟评估了系统发育分析、生理特性、抗原性和 B 细胞表位预测。我们报告了 325 例疑似患者的临床流行病学情况。其中，118 例（36.30%）通过 PCR 或 ELISA 确认为 CHIKV 阳性。临床分析显示关节疼痛、关节肿胀和头痛是常见且显著的特征。系统发育分析表明，目前流行的毒株与非洲、乌干达和新加坡的 CHIKV 株密切聚类。通过 WGS 进行了分子特征分析。发现非结构蛋白中的 38 个氨基酸发生了变化，与 S27（ECSA）株相比。其中 5 个位于 nsP2 中。同样，结构蛋白中观察到 34 个氨基酸变化。主要变化是 E3 蛋白的亲水性从-0.281 到-0.362，E2 的等电点（pI）从 8.24 到 8.37，不稳定性指数从 66.08 到 71.062，脂肪指数从 74.69 到 68.59，E3 从 75.79 到 70.05。在 nsP1 蛋白中，pI 从 6.62 到 8.04 不等，而在结构和非结构蛋白中没有观察到其他变化。线性 B 细胞表位的位置和数量随突变而变化。WGS 的分子特征表明，观察到了与突变相关的蛋白、抗原性。
