Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.
Faculty of Environment and Resource Studies, Mahidol University, Nakhon Pathom 73170, Thailand.
Bioresour Technol. 2021 Feb;322:124533. doi: 10.1016/j.biortech.2020.124533. Epub 2020 Dec 13.
Third generation biomass, i.e. microalgae, has emerged as a promising alternative to first and second generation biomass for biohydrogen production. However, its utilization is still low at present, due to several reasons including the strong and rigidity of the microalgal cell wall that limit the hydrolysis efficiency during dark fermentation (DF) and photofermentation (PF) processes. To improve the utilization efficiency of microalgal biomass, it is crucial that important aspects related to the production of the biomass and the following processes are elaborated. Thus, this article provides detailed overview of algal strains, cultivation, and harvesting. It also presents recent research and detailed information on microalgal biomass pretreatment, and biohydrogen production through DF, PF, and co-digestion of microalgal biomass with organic materials. Furthermore, factors affecting fermentation processes performance and the use of molecular techniques in biohydrogen production are presented. This review also discusses challenges and future prospects towards biohydrogen production from microalgal biomass.
第三代生物质，即微藻，已成为生物制氢生产中第一代和第二代生物质的有前途的替代品。然而，由于微藻细胞壁的强刚性限制了暗发酵（DF）和光发酵（PF）过程中的水解效率等多种原因，其利用率目前仍然较低。为了提高微藻生物质的利用效率，详细阐述与生物质生产和后续过程相关的重要方面至关重要。因此，本文详细介绍了藻种、培养和收获。它还介绍了通过 DF、PF 以及微藻生物质与有机材料共消化生产生物氢的最新研究和详细信息。此外，还介绍了影响发酵过程性能的因素以及分子技术在生物制氢中的应用。本文还讨论了从微藻生物质生产生物氢面临的挑战和未来前景。
