School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
Department of Environmental Energy Engineering, Kyonggi University, 94 San, Iui-dong, Youngtong-gu, Suwon-si, Gyeonggi-do 16227, Republic of Korea.
Bioresour Technol. 2018 Jul;260:16-22. doi: 10.1016/j.biortech.2018.03.029. Epub 2018 Mar 21.
This study assessed the impact of swine manure (SM) dilution ratio on the microalgal biomass cultivation and further tested for biohydrogen production efficiency from the mixed microalgal biomass. At first, various solid/liquid (S/L) ratio of the SM ranged from 2.5 to 10 g/L was prepared as a nutrient medium for the algal biomass cultivation without addition of the external nutrient sources over a period of 18 d. The peak biomass concentration of 2.57 ± 0.03 g/L was obtained under the initial S/L loading rates of 5 g/L. Further, the cultivated biomass was subjected to two-step (ultrasonication + enzymatic) pretreatment and evaluated for biohydrogen production potential. Results showed that the variable amount of hydrogen production was observed with different S/L ratio of the SM. The peak hydrogen yield of 116 ± 6 mL/g TS was observed at the 5 g/L grown SM mixed algal biomass.
本研究评估了猪粪（SM）稀释率对微藻生物量培养的影响，并进一步测试了混合微藻生物质的生物制氢效率。首先，将不同的固/液（S/L）比例的 SM（范围为 2.5 至 10 g/L）制备为营养培养基，在 18 d 的时间内无需添加外部营养源即可培养藻类生物质。在初始 S/L 加载率为 5 g/L 的情况下，获得了 2.57±0.03 g/L 的峰值生物量浓度。进一步地，将培养的生物质进行两步（超声处理+酶处理）预处理，并评估其生物制氢潜力。结果表明，不同 SM 的 S/L 比例会导致产氢量的变化。在 5 g/L 生长的 SM 混合藻生物质中，观察到 116±6 mL/g TS 的峰值氢气产量。
