Guangdong Key Laboratory of Fermentation and Enzyme Engineering, School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China.
State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, PR China.
Bioresour Technol. 2020 Apr;302:122879. doi: 10.1016/j.biortech.2020.122879. Epub 2020 Jan 23.
A cost-effective and environmentally friendly method for biofuel production was developed, by utilizing duckweed as feedstock for biohydrogen production through dark fermentation and simultaneously using the fermentative waste to produce microalgal lipids. The results suggested that acid hydrolysis (1% HSO) was more suitable for the pretreatment of duckweed biomass. Maximum hydrogen production of 169.30 mL g dry weight was determined under a temperature of 35 °C and an initial pH of 7.0. After the dark fermentation, the volatile fatty acids (VFAs) including acetate and butyrate, were detected in the waste, with concentration determined as 1.04 g L and 1.52 g L, respectively. During the mixotrophic cultivation of Chlorella sacchrarophila FACHB-4 using waste as feedstock, the maximum microalgal biomass and the lipid productions were about 2.8 and 33 times higher with respect to the autotrophic growth. The simultaneous biohydrogen production and waste utilization method provided a green strategy for biofuel production.
开发了一种经济高效且环保的生物燃料生产方法，利用浮萍作为生物制氢的原料，通过黑暗发酵生产生物氢，同时利用发酵废物生产微藻油脂。结果表明，酸水解（1% HSO）更适合浮萍生物质的预处理。在温度为 35°C 和初始 pH 值为 7.0 的条件下，确定最大产氢量为 169.30 毫升克干重。黑暗发酵后，废水中检测到乙酸和丁酸等挥发性脂肪酸（VFAs），浓度分别为 1.04 克升和 1.52 克升。在利用废物作为饲料进行混养培养时，与自养生长相比，最大微藻生物量和油脂产量分别提高了约 2.8 倍和 33 倍。同时进行生物制氢和废物利用的方法为生物燃料生产提供了一种绿色策略。
