Centro de Microscopia Eletronica, Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Campus Soane Nazare de Andrade, Ilheus, Brazil.
Hospital Veterinario, Departamento de Ciencias Agrarias e Ambientais, Universidade Estadual de Santa Cruz, Campus Soane Nazare de Andrade, Ilheus, Brazil.
Biol Reprod. 2021 Mar 11;104(3):548-561. doi: 10.1093/biolre/ioaa229.
Failures in hypothalamic kisspeptin/Kiss1r signaling are associated with infertility, and in vitro studies have shown that kisspeptin can modulate angiogenesis and immune activity. Because there is no in vivo research on the functional relationship between these factors in the reproductive system, especially in domestic cats, we evaluated the expression profile of kisspeptin/Kiss1r and angiogenic and immunological mediators in the genital tract of cyclic cats and of those with pyometra. The uterus of cats in diestrus exhibited greater gene and protein expression of Kiss1, as well as Vegf, Pigf, Mif, and Il6. In contrast, Kiss1r presented greater expression in proestrus/estrus, similarly to that observed for the immunostaining of INFγ, MIF, TNFα, and IL10. These factors were positively correlated with Kiss1 and/or Kiss1r, and a positive correlation between Kiss1 and Kiss1r was also observed in the uterus of cats during the estrous cycle. Cats with pyometra showed greater immunostaining of Kiss1 and Kiss1r on the endometrial surface and reduced immunostaining of Kiss1 in deep glands, whereas there was a significant reduction in Vegf, Pigf, Mif, and Il6 mRNA, and an increase in Tnf mRNA. The findings reveal that there is a gene correlation between kisspeptin/Kiss1r and angiogenic and immune mediators in the uterus of the domestic cat, which is modulated by the estrous cycle, and that pyometra affects the expression of these mediators. This study suggests, for the first time, a functional relationship between the Kiss/Kiss1r system and angiogenic and immune mediators in the female genital tract.
下丘脑 kisspeptin/Kiss1r 信号的失败与不孕有关，体外研究表明 kisspeptin 可以调节血管生成和免疫活性。由于在生殖系统中，特别是在家猫中，这些因素之间的功能关系没有体内研究，我们评估了生殖道中 kisspeptin/Kiss1r 和血管生成及免疫介质的表达谱在发情周期的猫和脓毒症的猫。发情期的猫的子宫表现出 Kiss1、Vegf、Pigf、Mif 和 Il6 的基因和蛋白表达增加。相反，Kiss1r 在发情前期/发情期表达增加，与 IFNγ、MIF、TNFα 和 IL10 的免疫染色观察结果相似。这些因子与 Kiss1 和/或 Kiss1r 呈正相关，并且在发情周期的猫的子宫中也观察到 Kiss1 与 Kiss1r 之间存在正相关。患有子宫积脓的猫的子宫内膜表面 Kiss1 和 Kiss1r 的免疫染色增加，而深部腺体的 Kiss1 免疫染色减少，而 Vegf、Pigf、Mif 和 Il6 mRNA 的表达显著减少，Tnf mRNA 增加。这些发现表明，家猫子宫中 kisspeptin/Kiss1r 与血管生成和免疫介质之间存在基因相关性，这种相关性受发情周期调节，而子宫积脓会影响这些介质的表达。本研究首次表明 Kiss/Kiss1r 系统与女性生殖道中的血管生成和免疫介质之间存在功能关系。
