Department of Respiratory Medicine, The Second Hospital of Jilin University, Changchun, China.
Stem Cells Dev. 2021 Feb;30(3):128-134. doi: 10.1089/scd.2020.0174. Epub 2021 Jan 18.
Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a severe clinical respiratory failure disorder associated with chronic pathology and disability and has a mortality rate of 40%-60%. However, the pathogenesis of ARDS/ALI remains unclear, and existing therapeutic options are insufficient for addressing the severity of the disease. Mesenchymal stem cells (MSCs) play an important role in the prevention and treatment of ALI, especially acute alveolar epithelial injury. However, the low survival rate of transplanted MSCs reduces their effectiveness. When human umbilical cord MSCs (hUC-MSCs) are transplanted directly, only a minority of cells migrate toward damaged tissues. Moreover, their maintenance time is short, leading to unsatisfactory therapeutic results. A moderate hypoxic environment can promote the proliferation of MSCs, inhibit apoptosis, and facilitate migration and chemotaxis. In summary, hypoxic culturing before transplantation improves the effectiveness of hUC-MSCs in treating ARDS/ALI and promises to provide novel diagnostic and therapeutic targets.
急性呼吸窘迫综合征（ARDS）/急性肺损伤（ALI）是一种严重的临床呼吸衰竭疾病，与慢性病理和残疾有关，死亡率为 40%-60%。然而，ARDS/ALI 的发病机制仍不清楚，现有的治疗选择不足以应对疾病的严重程度。间充质干细胞（MSCs）在 ALI 的防治中起着重要作用，特别是急性肺泡上皮损伤。然而，移植的 MSCs 存活率低，降低了它们的疗效。当直接移植人脐带间充质干细胞（hUC-MSCs）时，只有少数细胞向受损组织迁移。此外，它们的维持时间很短，导致治疗效果不理想。适度的低氧环境可以促进 MSCs 的增殖，抑制细胞凋亡，并促进迁移和趋化性。综上所述，移植前的低氧培养提高了 hUC-MSCs 治疗 ARDS/ALI 的效果，有望为其提供新的诊断和治疗靶点。
