Akyash Fatemeh, Javidpou Mahdieh, Yazd Ehsan Farashahi, Golzadeh Jalal, Hajizadeh-Tafti Fatemeh, Aflatoonian Reza, Aflatoonian Behrouz
Stem Cell Biology Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Department of Reproductive Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Int J Reprod Biomed. 2020 Nov 22;18(11):943-950. doi: 10.18502/ijrm.v13i11.7961. eCollection 2020 Nov.
Human endometrium with consecutive regeneration capability undergoes monthly hormonal changes for probable implantation, which confirms the presence of the cells in the basalis layer known as stem cell.
Previously, we reported the isolation and culture of the mesenchymal-like cells from human endometrium. In this study, we evaluated the biological and stemness characteristics of these cells.
The characterization of Yazd human endometrial-derived mesenchymal stem/stromal cells (YhEnMSCs) was assessed using immunofluorescence (IF) staining for CD105, VIMENTIN, and FIBRONECTIN as markers and RT-PCR for , , and genes. Flow cytometry (FACS) was performed for CD44, CD73, CD90, and CD105 markers. Moreover, the differentiation capacity of the YhEnMSCs to the osteoblast and adipocytes was confirmed by Alizarin Red and Oil Red staining.
YhEnMSCs expressed CD105, VIMENTIN, FIBRONECTIN, CD44, CD73, and CD90 markers and and but, did not express , .
Our data confirm previous reports by other groups indicating the application of endometrial cells as an available source of MSCs with self-renewal and differentiation capacity. Accordingly, YhEnMSCs can be used as a suitable source for cell-based therapies.
具有连续再生能力的人类子宫内膜每月会经历激素变化以利于可能的着床，这证实了基底层中存在被称为干细胞的细胞。
此前，我们报道了从人类子宫内膜中分离和培养间充质样细胞。在本研究中，我们评估了这些细胞的生物学特性和干性特征。
使用免疫荧光（IF）染色检测CD105、波形蛋白和纤连蛋白作为标志物，并通过逆转录聚合酶链反应（RT-PCR）检测 、 和 基因，以此对亚兹德人类子宫内膜来源的间充质干/基质细胞（YhEnMSCs）进行表征。对CD44、CD73、CD90和CD105标志物进行流式细胞术（FACS）检测。此外，通过茜素红和油红染色证实YhEnMSCs向成骨细胞和脂肪细胞的分化能力。
YhEnMSCs表达CD105、波形蛋白、纤连蛋白、CD44、CD73和CD90标志物以及 和 ，但不表达 、 。
我们的数据证实了其他研究小组之前的报道，即子宫内膜细胞可作为具有自我更新和分化能力的间充质干细胞的可用来源。因此，YhEnMSCs可作为基于细胞治疗的合适来源。
