Momeni Hamid Reza, Abnosi Mohammad Hussein, Eskandari Najmeh
Department of Biology, Faculty of Science, Arak University, Arak, Iran.
Int J Reprod Biomed. 2020 Nov 22;18(11):983-988. doi: 10.18502/ijrm.v13i11.7966. eCollection 2020 Nov.
3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay which evaluates cellular mitochondrial activity is widely used for the assessment of cell proliferation and viability.
This study was performed to assess human sperm viability using MTT assay.
In this laboratory study, human-ejaculated semen samples (n = 56 from different donors) were used. The sperm viability was determined using quantitative MTT assay and the sperm motility was assessed according to World Health Organization guidelines. Sperm viability and the correlation between sperm viability and motility were analyzed.
Data revealed a marked positive correlation between MTT reduction rate and the percentage of viable spermatozoa. The Pearson's correlation coefficients also showed a significant correlation between sperm viability and motility.
MTT assay which is based on mitochondrial functionality is a reliable method for evaluating human sperm viability and could be used as a diagnostic test for predicting sperm fertilization ability in clinical settings.
评估细胞线粒体活性的3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法广泛用于评估细胞增殖和活力。
本研究旨在使用MTT法评估人类精子活力。
在本实验室研究中，使用了人类射出的精液样本（来自不同供体，n = 56）。使用定量MTT法测定精子活力，并根据世界卫生组织指南评估精子活力。分析精子活力以及精子活力与活力之间的相关性。
数据显示MTT还原率与活精子百分比之间存在显著正相关。皮尔逊相关系数也显示精子活力与活力之间存在显著相关性。
基于线粒体功能的MTT法是评估人类精子活力的可靠方法，可作为临床环境中预测精子受精能力的诊断测试。
