Kooli Rim, Boussabbeh Manel, Chebil Dhekra, Kenani Abderraouf, Khefacha Linda, Mehdi Meriem, Sallem Amira
Department of Reproductive Biology, Maternity and Neonatology Center, Fattouma Bourguiba University Teaching Hospital, Monastir, Tunisia.
Laboratory of Research on Biologically Compatible Substances, Faculty of Dentistry of Monastir, Monastir University, Monastir, Tunisia.
PLoS One. 2025 Apr 28;20(4):e0319471. doi: 10.1371/journal.pone.0319471. eCollection 2025.
Platelet-rich plasma (PRP) is a pioneering therapy widely used in various medical fields, showing promising outcomes. However, its impact on human sperm quality remains poorly explored among emerging therapies. This study aims to investigate the effect of autologous PRP supplementation on oxidative stress levels and mitochondrial activity in human sperm. PRP was freshly prepared from venous blood and added to each ejaculated semen sample at different concentrations of 2%, 5%, and 10%. Reactive oxygen species (ROS) in spermatozoa were measured after 24 hours of incubation at 37° (5% CO2), using nitro-blue tetrazolium (NBT) test. The MTT test was used to measure the mitochondrial succinate deshydrogenase activity. A total of 180 semen samples were obtained from 15 patients. The supplementation with PRP significantly reduced the reactive oxidative species levels and improved mitochondrial activity in spermatozoa. The level of oxidative stress in sperm was significantly decreased after 24h of incubation with PRP at 2% (p = 0.001), 5% (p = 0.001) and 10% (p = 0.001) when compared to the control group. The succinate dehydrogenase activity was enhanced in the three groups when compared to the control group. It increased from 0.667 ± 0.313 to 0.952 ± 0.499 (p = 0.018), 1.201 ± 0.657 (p = 0.002) and 1.159 ± 0.607 (p = 0.001) after incubation with 2%, 5% and 10% of PRP, respectively. This study has shown that PRP supplementation could be a promising tool to enhance sperm quality against oxidative stress and mitochondrial dysfunction. These findings could be a starting point to investigate the usefulness of PRP in ART procedures.
富血小板血浆（PRP）是一种在各个医学领域广泛应用的开创性疗法，已显示出有前景的疗效。然而，在新兴疗法中，其对人类精子质量的影响仍未得到充分研究。本研究旨在探讨自体PRP补充对人类精子氧化应激水平和线粒体活性的影响。PRP由静脉血新鲜制备，并以2%、5%和10%的不同浓度添加到每份射精精液样本中。在37°（5%二氧化碳）孵育24小时后，使用硝基蓝四氮唑（NBT）试验测量精子中的活性氧（ROS）。MTT试验用于测量线粒体琥珀酸脱氢酶活性。共从15名患者中获取了180份精液样本。PRP补充显著降低了精子中的活性氧化物质水平，并改善了精子的线粒体活性。与对照组相比，在2%（p = 0.001）、5%（p = 0.001）和10%（p = 0.001）的PRP孵育24小时后，精子中的氧化应激水平显著降低。与对照组相比，三组中的琥珀酸脱氢酶活性均增强。与2%、5%和10%的PRP孵育后，其分别从0.667±0.313增加到0.952±0.499（p = 0.018）、1.201±0.657（p = 0.002）和1.159±0.607（p = 0.001）。本研究表明，PRP补充可能是一种有前景的工具，可提高精子质量，对抗氧化应激和线粒体功能障碍。这些发现可能是研究PRP在辅助生殖技术（ART）程序中的有用性的起点。
