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铁（III）、镍（II）及其混合物处理后正常成纤维细胞和肝癌细胞的细胞活力

Cell Viability in Normal Fibroblasts and Liver Cancer Cells After Treatment with Iron (III), Nickel (II), and their Mixture.

作者信息

Terpiłowska Sylwia, Siwicka-Gieroba Dorota, Siwicki Andrzej Krzysztof

机构信息

Laboratory of Environmental Biology, Institute of Environmental Engineering, The John Paul II Catholic University of Lublin, 20-950 Lublin, Poland.

Department of Anesthesiology and Intensive Care, Faculty of Medicine, Medical University in Lublin, 20-850 Lublin, Poland.

出版信息

J Vet Res. 2018 Dec 31;62(4):535-542. doi: 10.2478/jvetres-2018-0067. eCollection 2018 Dec.

DOI:10.2478/jvetres-2018-0067
PMID:30729213
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6364161/
Abstract

INTRODUCTION

Nickel and iron are very commonly occurring metals. Nickel is used in industry, but nowadays it is also used in medical biomaterials. Iron is an element necessary for cell metabolism and is used in diet supplements and biomaterials, whence it may be released along with nickel.

MATERIAL AND METHODS

BALB/3T3 and HepG2 cells were incubated with iron chloride or nickel chloride at concentrations ranging from 100 to 1,400 μM. The following mixtures were used: iron chloride 200 μM plus nickel chloride 1,000 μM, or iron chloride 1,000 μM plus nickel chloride 200 μM. The cell viability was determined with MTT, LHD, and NRU tests.

RESULTS

A decrease in cell viability was observed after incubating the BALB/3T3 and HepG2 cells with iron chloride or nickel chloride. A synergistic effect was observed after iron chloride 1,000 μM plus nickel chloride 200 μM treatment in all assays. Moreover, the same effect was observed in the pair iron chloride 200 μM plus nickel chloride 1,000 μM in the LDH and NRU assays.

CONCLUSIONS

Iron (III) and nickel (II) decrease cell viability. Iron chloride at a concentration of 200 μM protects mitochondria from nickel chloride toxicity.

摘要

引言

镍和铁是非常常见的金属。镍用于工业，但如今也用于医用生物材料。铁是细胞代谢所必需的元素，用于饮食补充剂和生物材料，因此它可能会与镍一起释放出来。

材料与方法

将BALB/3T3细胞和HepG2细胞与浓度范围为100至1400μM的氯化铁或氯化镍一起孵育。使用了以下混合物：200μM氯化铁加1000μM氯化镍，或1000μM氯化铁加200μM氯化镍。通过MTT、LHD和NRU试验测定细胞活力。

结果

在用氯化铁或氯化镍孵育BALB/3T3细胞和HepG2细胞后，观察到细胞活力下降。在所有试验中，用1000μM氯化铁加200μM氯化镍处理后观察到协同效应。此外，在LDH和NRU试验中，在200μM氯化铁加1000μM氯化镍这一组中也观察到了相同的效应。

结论

铁（III）和镍（II）会降低细胞活力。200μM浓度的氯化铁可保护线粒体免受氯化镍的毒性作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4131/6364161/c86681c5ead4/jvetres-62-535-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4131/6364161/f1c72a76fa55/jvetres-62-535-g001.jpg
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