CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Changsha, China.
University of Chinese Academy of Sciences, Beijing, China.
J Sci Food Agric. 2021 Aug 15;101(10):4018-4032. doi: 10.1002/jsfa.11037. Epub 2021 Jan 22.
As an enzymatic product of yeast, yeast-based nucleotide (YN) is rich in nucleotides. To test the effects of maternal dietary supplementation with YN during late pregnancy on placental nutrient transport and nutrient metabolism in neonatal piglets, 64 pregnant sows (day 85 ± 3) were assigned into two groups: (i) control (CON) and (ii) treatment (YN; 4 g kg ). Blood, placenta and liver samples of neonates during delivery were collected.
The results showed that maternal YN supplementation decreased stillbirth rate and intra-uterine growth restriction rate (P < 0.05). In addition, maternal YN supplementation increased total serum protein, albumin and total cholesterol (P < 0.05). Furthermore, in neonatal piglets in the YN group, both serum amino acidand nucleotide profiles were affected, as well as liver amino acid, and fatty acid profiles were regulated (P < 0.05). Moreover, maternal YN supplementation increased liver mRNA expression of SLC28A3, SLC29A1, SLC29A2, PC, PCK1, FBP1, SREBP1c, HSL and CYP7a1 of neonatal piglets (P < 0.05). Meanwhile, there was a decrease in placental gene expression of EAAT2, EAAT3, LAT1 and PAT1, as well as lower protein expression of peroxisome proliferator-activated receptor (PPAR)γ, AKT, phosphorylated-AKT, phosphorylated-mammalian target of rapamycin (mTOR) and Raptor, in the YN group (P < 0.05).
Taken together, these results indicate that maternal YN supplementation regulates placental nutrient transport by regulating the mTOR complex 1-PPAR pathway, and affects the liver metabolism of nucleotides, amino acids and fatty acids in neonatal piglets, thereby improving the reproductive performance of sow to a certain extent. © 2020 Society of Chemical Industry.
作为酵母的酶解产物，酵母核苷酸（YN）富含核苷酸。为了检测妊娠晚期母体补充 YN 对新生仔猪胎盘营养转运和营养代谢的影响，将 64 头妊娠母猪（第 85±3 天）分为 2 组：（i）对照组（CON）和（ii）处理组（YN；4 g·kg-1）。在分娩时收集新生仔猪的血液、胎盘和肝脏样本。
结果表明，母体 YN 补充降低了死产率和宫内生长受限率（P<0.05）。此外，母体 YN 补充增加了总血清蛋白、白蛋白和总胆固醇（P<0.05）。此外，YN 组新生仔猪的血清氨基酸和核苷酸谱以及肝脏氨基酸和脂肪酸谱均受到影响（P<0.05）。此外，母体 YN 补充增加了新生仔猪肝脏 SLC28A3、SLC29A1、SLC29A2、PC、PCK1、FBP1、SREBP1c、HSL 和 CYP7a1 的 mRNA 表达（P<0.05）。同时，YN 组胎盘 EAAT2、EAAT3、LAT1 和 PAT1 的基因表达下降，过氧化物酶体增殖物激活受体（PPAR）γ、AKT、磷酸化-AKT、磷酸化-雷帕霉素靶蛋白（mTOR）和 Raptor 的蛋白表达降低（P<0.05）。
综上所述，这些结果表明，母体 YN 补充通过调节 mTOR 复合物 1-PPAR 途径调节胎盘营养转运，并影响新生仔猪肝脏核苷酸、氨基酸和脂肪酸的代谢，从而在一定程度上提高母猪的繁殖性能。 © 2020 英国化学学会。
