1New York State Department of Health, The Arbovirus Laboratory, Wadsworth Center, Slingerlands, New York.
2New York State Department of Health, Bureau of Communicable Disease Control, Albany, New York.
Am J Trop Med Hyg. 2020 Dec 21;104(2):593-603. doi: 10.4269/ajtmh.20-1090.
Sera from white-tailed deer (WTD, Odocoileus virginianus) hunter-harvested throughout New York State (NYS), 2007-2015, were tested by plaque reduction neutralization for antibodies against nine mosquito-borne viruses from the families Peribunyaviridae, Flaviviridae, and Togaviridae. Overall, 76.1% (373/490) of sampled WTD were seropositive against at least one virus, and 38.8% were exposed to multiple viruses. The seropositivity rate in adult WTD (78.0%) was significantly greater (P < 0.0001) than that in fawns (47.7%). Neutralizing antibodies against California serogroup viruses were most common in WTD sampled across all regions (67.1%), followed by the Bunyamwera serogroup (BUN) (37.6%). Jamestown Canyon and Cache Valley orthobunyaviruses were responsible for most California and BUN infections, respectively. Seroprevalence rates to West Nile virus were higher in samples originating from Long Island (LI) (19.0%) than in those originating from the central (7.3%), western (5.0%), and Hudson Valley (4.4%) regions of NYS. Antibodies to Eastern equine encephalitis virus were seen primarily in WTD from central NYS (5.1%), where annual enzootic activity occurs, but low rates were documented in western NYS (1.4%) and LI (1.7%). Low rates of Potosi and LaCrosse orthobunyavirus, and Highlands J virus antibodies were detected over the course of this investigation. St. Louis encephalitis virus (or a closely related virus) antibodies were detected in samples collected from central and western NYS, suggesting local virus transmission despite a lack of evidence from routine mosquito surveillance. Serologic results demonstrate the value of WTD in NYS as an indicator of arbovirus distribution and recent transmission on a relatively fine spatial scale.
2007-2015 年期间，从美国纽约州（NYS）各地猎捕的白尾鹿（Odocoileus virginianus）血清被检测出针对披膜病毒科、黄病毒科和正粘病毒科的 9 种虫媒病毒的抗体，采用噬斑减少中和试验进行检测。总体而言，490 份样本中有 76.1%（373/490）对至少一种病毒呈血清阳性，38.8%的样本暴露于多种病毒。成鹿（78.0%）的血清阳性率明显高于幼鹿（47.7%）（P<0.0001）。在所有地区采样的白尾鹿中，针对加利福尼亚血清群病毒的中和抗体最为常见（67.1%），其次是 Bunyamwera 血清群（BUN）（37.6%）。Jamestown Canyon 和 Cache Valley Orthobunyavirus 分别是加利福尼亚和 BUN 感染的主要原因。在长岛（LI）来源的样本中，西尼罗河病毒的血清阳性率（19.0%）高于来自 NYS 中心（7.3%）、西部（5.0%）和哈德逊河谷（4.4%）地区的样本。在 NYS 中心地区（5.1%）的白尾鹿中发现了东方马脑炎病毒的抗体，而在西部 NYS（1.4%）和 LI（1.7%）的白尾鹿中发现了低水平的抗体。在此调查过程中，检测到了低水平的 Potosi 和 LaCrosse Orthobunyavirus 和 Highlands J 病毒抗体。在从 NYS 中心和西部采集的样本中检测到了圣路易斯脑炎病毒（或密切相关的病毒）抗体，表明尽管缺乏常规蚊虫监测的证据，但仍存在当地病毒传播。血清学结果表明，白尾鹿在 NYS 作为指示病毒分布和最近在相对精细的空间尺度上传播的指标具有重要价值。
