Integral University, Faculty of Pharmacy, Kursi Road, Lucknow 226026, India.
School of Pharmaceutical Education & Research Jamia Hamdard, Department of Pharmaceutics, New Delhi 110062, India.
J AOAC Int. 2021 Aug 20;104(4):1188-1195. doi: 10.1093/jaoacint/qsaa171.
The well-known medicinal plants Ricinus communis L. and Euphorbia hirta L. have been traditionally used to treat a variety of ailments in the India and other countries.
In the present research, a simple, rapid, reliable, and accessible HPTLC technique has been established for concurrent quantification of phenolic compounds (syringic acid and vanillic acid) and evaluation of their anti-oxidant capacity.
The chromatographic separation was conceded on pre-coated silica gel plates 60 F254 as the stationary phase. The appropriate mobile phase combination of toluene-ethyl acetate-formic acid (7:2.5:0.5, v/v/v) were developed to expand the plates which separated components according to the marker compounds. Further, the anti-oxidant potential of methanol extract of R. communis (MERC) and E. hirta (MERH) were also assessed with 1,1-diphenyl-2-picrylhydrazyl (DPPH) by using UV spectrophotometry.
Densitometric scanning was performed with a Camag V scanner and measured at two different wave lengths, 272 and 318 nm. The marker compounds were practically resolved with RF 0.5 ± 0.04 for syringic acid and 0.6 ± 0.06 for vanillic acid. The results obtained in the study of anti-oxidant activity of MERC and MERH showed significant free radical scavenging capacity against DPPH-generated free radicals.
The developed HPTLC method was validated for accuracy, linearity, precision, and specificity. Both the extracts revealed considerable antioxidant activity. The reported existing phenolic and flavonoids compounds are responsible for antioxidant activity of plant extracts.
蓖麻（Ricinus communis L.）和飞扬草（Euphorbia hirta L.）是众所周知的药用植物，在印度和其他国家传统上被用于治疗多种疾病。
本研究建立了一种简单、快速、可靠且易于使用的 HPTLC 技术，用于同时定量测定酚类化合物（丁香酸和香草酸）并评估其抗氧化能力。
采用涂覆有硅胶 60 F254 的预制板作为固定相进行色谱分离。开发了甲苯-乙酸乙酯-甲酸（7:2.5:0.5，v/v/v）的适当流动相组合，将根据标记化合物分离的板展开。此外，还使用 1,1-二苯基-2-苦基肼（DPPH）通过紫外分光光度法评估了蓖麻甲醇提取物（MERC）和飞扬草甲醇提取物（MERH）的抗氧化潜力。
使用 Camag V 扫描仪进行密度扫描，并在 272 和 318nm 两个不同波长下进行测量。标记化合物实际上在 0.5±0.04 处与丁香酸分离，在 0.6±0.06 处与香草酸分离。MERC 和 MERH 抗氧化活性研究的结果表明，它们对 DPPH 产生的自由基具有显著的清除能力。
所开发的 HPTLC 方法已针对准确性、线性、精密度和特异性进行了验证。两种提取物均表现出相当大的抗氧化活性。报告中存在的酚类和类黄酮化合物是植物提取物抗氧化活性的原因。
