Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam - 632 509, Vellore District, Tamil Nadu, India.
Parasitol Res. 2012 Oct;111(4):1629-35. doi: 10.1007/s00436-011-2589-8. Epub 2011 Aug 14.
The purpose of this study is to determine the efficacies of hexane, chloroform, ethyl acetate, acetone, and methanol leaf extracts of Euphorbia hirta L., Psidium guajava L., Ricinus communis L., Solanum trilobatum L., and Tridax procumbens L. against sheep fluke Paramphistomum cervi (Digenea: Paramphistomatidae). All plant extracts showed moderate effects after 24 h of exposure; however, the highest parasite mortality was found in the methanol extract of R. communis. In the present study, bioassay-guided fractionation of methanol extract of R. communis led to the separation and identification of epicatechin as a potential new compound (LC(50) = 31.2; LC(90) = 105.0 ppm) against P. cervi. The structures were established from infrared, ultraviolet, (1)H-nuclear magnetic resonance (NMR), (13)C-NMR, and mass spectral data which confirmed the identification of the compound epicatechin from R. communis. Results of this study showed that the methanol extract of R. communis may be considered as a potent source and epicatechin as a new natural parasitic agent.
本研究旨在确定大戟（Euphorbia hirta L.）、番石榴（Psidium guajava L.）、蓖麻（Ricinus communis L.）、三叶鬼针草（Solanum trilobatum L.）和三脉紫菀（Tridax procumbens L.）的叶的正己烷、氯仿、乙酸乙酯、丙酮和甲醇提取物对绵羊双腔吸虫（Paramphistomum cervi（Digenea：Paramphistomatidae））的疗效。所有植物提取物在暴露 24 小时后均显示出中等作用；然而，在蓖麻的甲醇提取物中发现了最高的寄生虫死亡率。在本研究中，基于生物测定的蓖麻甲醇提取物的分步分离和鉴定得到表儿茶素，这是一种针对 P. cervi 的潜在新型化合物（LC(50) = 31.2；LC(90) = 105.0 ppm）。该化合物的结构是根据红外、紫外、（1）H-核磁共振（NMR）、（13）C-NMR 和质谱数据确定的，这些数据证实了化合物表儿茶素来自蓖麻。本研究结果表明，蓖麻甲醇提取物可被视为一种有效的天然驱虫药。
