School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
Biomed Res Int. 2021 Aug 24;2021:9490162. doi: 10.1155/2021/9490162. eCollection 2021.
() has anti-inflammatory, antinociceptive, and antipyretic activities; however, there are no literature reports on its antioxidant capacity. This study presents a comparative assessment of the polyphenols contents, flavonoids contents, and antioxidant activity of the aqueous and methanol extracts of (ASI and MSI). Moreover, the expression of oxidative stress-related genes in HO-induced H9c2 cells pretreated with the MSI was measured by RT-qPCR, and furthermore, MSI were characterized by UHPLC-Q-Orbitrap-MS/MS. The results indicated that the MSI had higher antioxidant contents and antioxidant capacity, and MSI could inhibit HO-induced oxidative stress in H9c2 cells by activating the Nrf2/HO-1 pathway. UHPLC-Q-Orbitrap-MS/MS characterized 15 phenolic compounds from the MSI. In conclusion, has the potential antioxidant capacity.
() 具有抗炎、镇痛和退热作用;然而,目前尚无关于其抗氧化能力的文献报道。本研究比较了 () 的水提物和甲醇提物(ASI 和 MSI)的多酚含量、黄酮含量和抗氧化活性。此外,通过 RT-qPCR 测量了 HO 诱导的 H9c2 细胞中 MSI 预处理后与氧化应激相关的基因表达,并且通过 UHPLC-Q-Orbitrap-MS/MS 对 MSI 进行了表征。结果表明,MSI 具有更高的抗氧化含量和抗氧化能力,并且 MSI 可以通过激活 Nrf2/HO-1 通路来抑制 HO 诱导的 H9c2 细胞中的氧化应激。UHPLC-Q-Orbitrap-MS/MS 从 MSI 中鉴定出 15 种酚类化合物。总之,() 具有潜在的抗氧化能力。