School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China.
Int J Biol Macromol. 2021 Feb 15;170:447-458. doi: 10.1016/j.ijbiomac.2020.12.126. Epub 2020 Dec 19.
The objective of present research was to explore whether Sargassum fusiforme polysaccharide (SFP) could partly replace acarbose against type 2 diabetes in rats. Results indicated that SFP co-administered with low-dose acarbose intervention typically mitigated diabetic symptoms and serum profiles and exhibited better anti-diabetic effects than single acarbose treatment in controlling fasting blood glucose, improving insulin resistance and mitigating kidney injuries. The RT-qPCR analysis indicated that SFP co-administered with low-dose acarbose administration distinctly activated the IRS/PI3K/AKT signaling pathway compared with single acarbose treatment. Moreover, the co-administration also restrained liver fat accumulation via affecting the expression of HMGCR and SREBP-1c genes. In addition, the 16S rRNA gene sequencing analysis indicated that SFP co-administered with low-dose acarbose significantly restored beneficial composition of gut flora in diabetic rats, such as the increase of Muribaculaceae, Lachnospiraceae, Bifidobacterium, Ruminococcaceae_UCG-014, Ruminococcus_1, Romboutsia, Eggerthellaceae, Alistipes and Faecalibaculum, and the decrease of Escherichia-Shigella. These results suggested that SFP, the novel natural adjuvant of acarbose, displayed the desirable benefits in minimizing the dose of drug, while improving the anti-diabetic efficiency.
本研究旨在探索马尾藻多糖（SFP）是否可以部分替代阿卡波糖治疗大鼠 2 型糖尿病。结果表明，SFP 与低剂量阿卡波糖联合干预通常可减轻糖尿病症状和血清谱，并比单独使用阿卡波糖治疗在控制空腹血糖、改善胰岛素抵抗和减轻肾脏损伤方面具有更好的抗糖尿病效果。RT-qPCR 分析表明，与单独使用阿卡波糖治疗相比，SFP 与低剂量阿卡波糖联合给药明显激活了 IRS/PI3K/AKT 信号通路。此外，联合给药还通过影响 HMGCR 和 SREBP-1c 基因的表达来抑制肝脂肪堆积。此外，16S rRNA 基因测序分析表明，SFP 与低剂量阿卡波糖联合给药可显著恢复糖尿病大鼠肠道菌群的有益组成，如 Muribaculaceae、Lachnospiraceae、双歧杆菌、Ruminococcaceae_UCG-014、Ruminococcus_1、Romboutsia、Eggerthellaceae、Alistipes 和 Faecalibaculum 的增加，以及 Escherichia-Shigella 的减少。这些结果表明，SFP 作为阿卡波糖的新型天然佐剂，在降低药物剂量的同时，提高了抗糖尿病效率，具有良好的应用前景。
