State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
College of Medicine, Zhejiang University, Hangzhou, China.
Microb Biotechnol. 2024 Oct;17(10):e70016. doi: 10.1111/1751-7915.70016.
Previous reports have demonstrated that alcohol consumption significantly reduces the abundance of Lactobacillus in the gut. In this study, we selected five species of the genus Lactobacillus, commonly found in fermented foods, and acknowledged them as safe, edible, and effective in preventing or treating certain diseases, to evaluate their effects on alcoholic liver disease (ALD). By comparing the liver damage indices in each group, we found that the type strain of Lactobacillus helveticus (LH, ATCC 15009) had the most marked alleviating effect on ALD-induced liver injury. Furthermore, experiments combining microbiomics and metabolomics were conducted to explore the mechanisms underlying the hepatoprotective effects of LH. Finally, we discovered that LH mitigated ethanol-induced liver steatosis and inflammation in ALD mice by altering the structure and function of the gut microbiome, increasing intestinal levels of short-chain fatty acids (SCFAs), and enhancing gut barrier integrity. These findings suggest a potential strategy for the clinical management of patients with ALD.
先前的报告表明，饮酒会显著减少肠道中乳杆菌的丰度。在这项研究中，我们选择了五种常见于发酵食品中的乳杆菌属，这些菌种被认为是安全、可食用的，并且能够有效预防或治疗某些疾病，以此来评估它们对酒精性肝病（ALD）的影响。通过比较各组的肝损伤指标，我们发现瑞士乳杆菌（LH，ATCC 15009）的模式株对 ALD 引起的肝损伤具有最显著的缓解作用。此外，我们还进行了结合微生物组学和代谢组学的实验，以探索 LH 发挥肝保护作用的机制。最后，我们发现 LH 通过改变肠道微生物组的结构和功能、增加肠道短链脂肪酸（SCFA）水平以及增强肠道屏障完整性，减轻了乙醇诱导的 ALD 小鼠的肝脂肪变性和炎症。这些发现为 ALD 患者的临床管理提供了一种潜在策略。
