Frailty is an expression that reconciles and condenses loss of autonomy, both physical and cognitive decline and a wide spectrum of adverse outcomes due to aging. The decrease in physical and cognitive activity is associated with altered mitochondrial function, and energy loss and consequently morbidity and mortality. In this cross-sectional study, we evaluated the carnitine levels in frailty status. The mean serum concentrations of total carnitine (TC) were lower in frail elderly subjects than in prefrail ones ( = 0.0006), higher in frail vs. robust subjects ( < 0.0001), and higher in prefrail vs. robust subjects ( < 0.0001). The mean serum concentrations of free carnitine (FC) were lower in frail elderly subjects than in prefrail ones ( < 0.0001), lower in frail vs. robust subjects ( < 0.0001) and lower in prefrail vs. robust subjects ( = 0.0009). The mean serum concentrations of acylcarnitine (AC) were higher in frail elderly subjects than in prefrail ones ( = 0.054) and were higher in pre-frail vs. robust subjects ( = 0.0022). The mean urine concentrations of TC were lower in frail elderly subjects than in prefrail ones ( < 0.05) and lower in frail vs. robust subjects ( < 0.0001). The mean urine concentrations of free carnitine were lower in frail elderly vs. robust subjects ( < 0.05). The mean urine concentrations of acyl carnitines were lower in frail elderly subjects than those in both prefrail ( < 0.0001) and robust subjects ( < 0.0001). Conclusion: high levels of carnitine may have a favorable effect on the functional status and may treat the frailty status in older subjects.