Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Viruses. 2020 Dec 19;12(12):1470. doi: 10.3390/v12121470.
Worldwide, poultry industry suffers from infections caused by avian pathogenic . Therapeutic failure due to resistant bacteria is of increasing concern and poses a threat to human and animal health. This causes a high demand to find alternatives to fight bacterial infections in animal farming. Bacteriophages are being especially considered for the control of multi-drug resistant bacteria due to their high specificity and lack of serious side effects. Therefore, the study aimed on characterizing phages and composing a phage cocktail suitable for the prevention of infections with . Six phages were isolated or selected from our collections and characterized individually and in combination with regard to host range, stability, reproduction, and efficacy in vitro. The cocktail consisting of six phages was able to inhibit formation of biofilms by some strains but not by all. Phage-resistant variants arose when bacterial cells were challenged with a single phage but not when challenged by a combination of four or six phages. Resistant variants arising showed changes in carbon metabolism and/or motility. Genomic comparison of wild type and phage-resistant mutant E28.G28R3 revealed a deletion of several genes putatively involved in phage adsorption and infection.
在全球范围内，家禽养殖业受到禽致病性细菌感染的困扰。由于耐药细菌的治疗失败，人们越来越关注这一问题，这对人类和动物的健康构成了威胁。因此，人们迫切需要寻找替代方法来对抗动物养殖中的细菌感染。由于噬菌体具有高度特异性且副作用较小，因此它们被特别考虑用于控制多药耐药菌。因此，本研究旨在对噬菌体进行表征，并组成一种噬菌体鸡尾酒，以预防禽致病性细菌感染。从我们的收藏中分离或选择了六个噬菌体，并单独和组合进行了宿主范围、稳定性、繁殖和体外疗效的特征分析。由六个噬菌体组成的鸡尾酒能够抑制一些 菌株形成生物膜，但不能抑制所有菌株。当用单一噬菌体挑战细菌细胞时，会出现噬菌体抗性变体，但当用四种或六种噬菌体组合挑战时，则不会出现。出现的抗性变体显示出碳代谢和/或运动性的变化。野生型和噬菌体抗性突变体 E28.G28R3 的基因组比较显示，几个推测与噬菌体吸附和感染有关的基因缺失。
